4b1q: Difference between revisions

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[[~~Image~~:~~4b1q~~.~~jpg~~|~~left~~|~~200px~~]]

==NMR structure of the glycosylated conotoxin CcTx from Conus consors==

== Structural highlights ==

<table><tr><td colspan='2'>[[4b1q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conus_consors Conus consors]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1Q FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GXL:ALPHA-L-GALACTOPYRANOSE'>GXL</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b1q RCSB], [http://www.ebi.ac.uk/pdbsum/4b1q PDBsum]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/CEX_CONCN CEX_CONCN]] May specifically activate neuronal voltage-gated sodium channels (Nav) at the resting membrane potential. Causes a marked contraction and extension of the caudal and dorsal fins in fish and noticeable spontaneous contractions of isolated frog neuromuscular preparations.

== Publication Abstract from PubMed ==

The glycopeptide CcTx, isolated from the venom of the piscivorous cone snail Conus consors, belongs to the kappaA-family of conopeptides. These toxins elicit excitotoxic responses in the prey by acting on voltage-gated sodium channels. The structure of CcTx, a first in the kappaA-family, has been determined by high-resolution NMR spectroscopy together with the analysis of its O-glycan at Ser7. A new type of glycopeptide O-glycan core structure, here registered as core type 9, containing two terminal L-galactose units {alpha-L-Galp-(1-->4)-alpha-D-GlcpNAc-(1-->6)-[alpha-L-Galp-(1-->2)-beta-D-Galp-( 1-->3)-]alpha-D-GalpNAc-(1-->O)}, is highlighted. A sequence comparison to other putative members of the kappaA-family suggests that O-linked glycosylation might be more common than previously thought. This observation alone underlines the requirement for more careful and in-depth investigations into this type of post-translational modification in conotoxins.

~~{{STRUCTURE_4b1q| PDB=4b1q | SCENE= }}~~

Structure of the O-Glycosylated Conopeptide CcTx from Conus consors Venom.,Hocking HG, Gerwig GJ, Dutertre S, Violette A, Favreau P, Stocklin R, Kamerling JP, Boelens R Chemistry. 2013 Jan 14;19(3):870-9. doi: 10.1002/chem.201202713. Epub 2012 Dec, 27. PMID:23281027<ref>PMID:23281027</ref>

~~===NMR structure~~ of the ~~glycosylated conotoxin CcTx from Conus consors===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_23281027}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[4b1q]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Conus_consors Conus consors]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1Q OCA].~~

</StructureSection>

[[Category: Conus consors]]

[[Category: Boelens, R.]]

[[Category: Boelens, R]]

[[Category: Favreau, P.]]

[[Category: Favreau, P]]

[[Category: Gerwig, G J.]]

[[Category: Gerwig, G J]]

[[Category: Hocking, H G.]]

[[Category: Hocking, H G]]

[[Category: Kamerling, J P.]]

[[Category: Kamerling, J P]]

[[Category: Stocklin, R.]]

[[Category: Stocklin, R]]

[[Category: O-glycan]]

[[Category: Toxin]]

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@@ Line 1: / Line 1: @@
-[[Image:4b1q.jpg|left|200px]]
+==NMR structure of the glycosylated conotoxin CcTx from Conus consors==
+<StructureSection load='4b1q' size='340' side='right' caption='[[4b1q]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4b1q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conus_consors Conus consors]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1Q FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GXL:ALPHA-L-GALACTOPYRANOSE'>GXL</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b1q RCSB], [http://www.ebi.ac.uk/pdbsum/4b1q PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/CEX_CONCN CEX_CONCN]] May specifically activate neuronal voltage-gated sodium channels (Nav) at the resting membrane potential. Causes a marked contraction and extension of the caudal and dorsal fins in fish and noticeable spontaneous contractions of isolated frog neuromuscular preparations.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The glycopeptide CcTx, isolated from the venom of the piscivorous cone snail Conus consors, belongs to the kappaA-family of conopeptides. These toxins elicit excitotoxic responses in the prey by acting on voltage-gated sodium channels. The structure of CcTx, a first in the kappaA-family, has been determined by high-resolution NMR spectroscopy together with the analysis of its O-glycan at Ser7. A new type of glycopeptide O-glycan core structure, here registered as core type 9, containing two terminal L-galactose units {alpha-L-Galp-(1--&gt;4)-alpha-D-GlcpNAc-(1--&gt;6)-[alpha-L-Galp-(1--&gt;2)-beta-D-Galp-( 1--&gt;3)-]alpha-D-GalpNAc-(1--&gt;O)}, is highlighted. A sequence comparison to other putative members of the kappaA-family suggests that O-linked glycosylation might be more common than previously thought. This observation alone underlines the requirement for more careful and in-depth investigations into this type of post-translational modification in conotoxins.
-{{STRUCTURE_4b1q|  PDB=4b1q  |  SCENE=  }}
+Structure of the O-Glycosylated Conopeptide CcTx from Conus consors Venom.,Hocking HG, Gerwig GJ, Dutertre S, Violette A, Favreau P, Stocklin R, Kamerling JP, Boelens R Chemistry. 2013 Jan 14;19(3):870-9. doi: 10.1002/chem.201202713. Epub 2012 Dec, 27. PMID:23281027<ref>PMID:23281027</ref>
-===NMR structure of the glycosylated conotoxin CcTx from Conus consors===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_23281027}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[4b1q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conus_consors Conus consors]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1Q OCA].
+</StructureSection>
 [[Category: Conus consors]]
-[[Category: Boelens, R.]]
+[[Category: Boelens, R]]
-[[Category: Favreau, P.]]
+[[Category: Favreau, P]]
-[[Category: Gerwig, G J.]]
+[[Category: Gerwig, G J]]
-[[Category: Hocking, H G.]]
+[[Category: Hocking, H G]]
-[[Category: Kamerling, J P.]]
+[[Category: Kamerling, J P]]
-[[Category: Stocklin, R.]]
+[[Category: Stocklin, R]]
 [[Category: O-glycan]]
 [[Category: Toxin]]