3dad: Difference between revisions
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[[Image: | ==Crystal structure of the N-terminal regulatory domains of the formin FHOD1== | ||
<StructureSection load='3dad' size='340' side='right' caption='[[3dad]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3dad]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DAD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DAD FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FHOD1, FHOS, FHOS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dad OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dad RCSB], [http://www.ebi.ac.uk/pdbsum/3dad PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/da/3dad_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Formins induce the nucleation and polymerization of unbranched actin filaments. They share three homology domains required for profilin binding, actin polymerization, and regulation. Diaphanous-related formins (DRFs) are activated by GTPases of the Rho/Rac family, whose interaction with the N-terminal formin domain is thought to displace a C-terminal Diaphanous-autoregulatory domain (DAD). We have determined the structure of the N-terminal domains of FHOD1 consisting of a GTPase-binding domain (GBD) and the DAD-recognition domain FH3. In contrast to the formin mDia1, the FHOD1-GBD reveals a ubiquitin superfold as found similarly in c-Raf1 or PI3 kinase. This GBD is recruited by Rac and Ras GTPases in cells and plays an essential role for FHOD1-mediated actin remodeling. The FHOD1-FH3 domain is composed of five armadillo repeats, similarly to other formins. Mutation of one residue in the predicted DAD-interaction surface efficiently activates FHOD1 in cells. These results demonstrate that DRFs have evolved different molecular solutions to govern their autoregulation and GTPase specificity. | |||
The human formin FHOD1 contains a bipartite structure of FH3 and GTPase-binding domains required for activation.,Schulte A, Stolp B, Schonichen A, Pylypenko O, Rak A, Fackler OT, Geyer M Structure. 2008 Sep 10;16(9):1313-23. PMID:18786395<ref>PMID:18786395</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Fackler, O T | [[Category: Fackler, O T]] | ||
[[Category: Geyer, M | [[Category: Geyer, M]] | ||
[[Category: Pylypenko, O | [[Category: Pylypenko, O]] | ||
[[Category: Rak, A | [[Category: Rak, A]] | ||
[[Category: Schonichen, A | [[Category: Schonichen, A]] | ||
[[Category: Schulte, A | [[Category: Schulte, A]] | ||
[[Category: Stolp, B | [[Category: Stolp, B]] | ||
[[Category: Actin-binding]] | [[Category: Actin-binding]] | ||
[[Category: Armadillo repeat]] | [[Category: Armadillo repeat]] | ||
Revision as of 07:59, 12 November 2014
Crystal structure of the N-terminal regulatory domains of the formin FHOD1
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