2yq5: Difference between revisions
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[[ | ==Crystal Structure of D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus: NAD complexed form== | ||
<StructureSection load='2yq5' size='340' side='right' caption='[[2yq5]], [[Resolution|resolution]] 2.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2yq5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_delbrueckii_subsp._bulgaricus Lactobacillus delbrueckii subsp. bulgaricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YQ5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yq4|2yq4]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yq5 RCSB], [http://www.ebi.ac.uk/pdbsum/2yq5 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hydroxyacid dehydrogenases, responsible for the stereospecific conversion of 2-keto acids to 2-hydroxyacids in lactic acid producing bacteria, have a range of biotechnology applications including antibiotic synthesis, flavor development in dairy products and the production of valuable synthons. The genome of Lactobacillus delbrueckii ssp. bulgaricus, a member of the heterogeneous group of lactic acid bacteria, encodes multiple hydroxyacid dehydrogenases whose structural and functional properties remain poorly characterized. Here, we report the apo and coenzyme NAD(+) complexed crystal structures of the L. bulgaricusD-isomer specific 2-hydroxyacid dehydrogenase, D2-HDH. Comparison with closely related members of the NAD-dependent dehydrogenase family reveals that whilst the D2-HDH core fold is structurally conserved, the substrate-binding site has a number of non-canonical features that may influence substrate selection and thus dictate the physiological function of the enzyme. | |||
Structural characterization of a D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus.,Holton SJ, Anandhakrishnan M, Geerlof A, Wilmanns M J Struct Biol. 2012 Oct 27. pii: S1047-8477(12)00291-2. doi:, 10.1016/j.jsb.2012.10.009. PMID:23110853<ref>PMID:23110853</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Lactobacillus delbrueckii subsp. bulgaricus]] | [[Category: Lactobacillus delbrueckii subsp. bulgaricus]] | ||
[[Category: Anandhakrishnan, M | [[Category: Anandhakrishnan, M]] | ||
[[Category: Geerlof, A | [[Category: Geerlof, A]] | ||
[[Category: Holton, S J | [[Category: Holton, S J]] | ||
[[Category: Wilmanns, M | [[Category: Wilmanns, M]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
