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[[ | ==Solution Structure of Duplex DNA Containing a b-Carba-Fapy-dG Lesion== | ||
<StructureSection load='2lwm' size='340' side='right' caption='[[2lwm]], [[NMR_Ensembles_of_Models | 26 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lwm]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LWM FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LWM:[(1R,2S,4R)-4-{[2-AMINO-5-(FORMYLAMINO)-6-OXO-1,6-DIHYDROPYRIMIDIN-4-YL]AMINO}-2-HYDROXYCYCLOPENTYL]METHYL+DIHYDROGEN+PHOSPHATE'>LWM</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lwn|2lwn]], [[2lwo|2lwo]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lwm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lwm RCSB], [http://www.ebi.ac.uk/pdbsum/2lwm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The addition of hydroxyl radicals to the C8 position of guanine can lead to the formation of a 2,6-diamino-4-hydroxy-5-formamido-2'-deoxypyrimidine (Fapy-dG) lesion, whose endogenous levels in cellular DNA rival those of 8-oxo-7,8-dihydroxy-2'-deoxyguanosine. Despite its prevalence, the structure of duplex DNA containing Fapy-dG is unknown. We have prepared an undecameric duplex containing a centrally located beta-cFapy-dG residue paired to dC and determined its solution structure by high-resolution NMR spectroscopy and restrained molecular dynamic simulations. The damaged duplex adopts a right-handed helical structure with all residues in an anti conformation, forming Watson-Crick base pair alignments, and 2-deoxyribose conformations in the C2'-endo/C1'-exo range. The formamido group of Fapy rotates out of the pyrimidine plane and is present in the Z and E configurations that equilibrate with an approximate 2:1 population ratio. The two isomeric duplexes show similar lesion-induced deviations from a canonical B-from DNA conformation that are minor and limited to the central three-base-pair segment of the duplex, affecting the stacking interactions with the 5-lesion-neighboring residue. We discuss the implications of our observations for translesion synthesis during DNA replication and the recognition of Fapy-dG by DNA glycosylases. | |||
Solution structure of duplex DNA containing a beta-Carba-Fapy-dG lesion.,Lukin M, Zaliznyak T, Attaluri S, Johnson F, de Los Santos C Chem Res Toxicol. 2012 Nov 19;25(11):2423-31. doi: 10.1021/tx300290b. Epub 2012, Aug 29. PMID:22897814<ref>PMID:22897814</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Attaluri, S | [[Category: Attaluri, S]] | ||
[[Category: Johnson, F | [[Category: Johnson, F]] | ||
[[Category: Lukin, M | [[Category: Lukin, M]] | ||
[[Category: Santos, C de los | [[Category: Santos, C de los]] | ||
[[Category: Zalianyak, T | [[Category: Zalianyak, T]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Oxidative damage]] | [[Category: Oxidative damage]] | ||
Revision as of 08:50, 26 November 2014
Solution Structure of Duplex DNA Containing a b-Carba-Fapy-dG Lesion
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