2xhz: Difference between revisions
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[[ | ==PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY== | ||
<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XHZ FirstGlance]. <br> | |||
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lipopolysaccharide (LPS) biosynthesis represents an underexploited target pathway for novel antimicrobial development to combat the emergence of multidrug-resistant bacteria. A key player in LPS synthesis is the enzyme D-arabinose-5-phosphate isomerase (API), which catalyzes the reversible isomerization of D-ribulose-5-phosphate to D-arabinose-5-phosphate, a precursor of 3-deoxy-D-manno-octulosonate that is an essential residue of the LPS inner core. API is composed of two main domains: an N-terminal sugar isomerase domain (SIS) and a pair of cystathionine-beta-synthase domains of unknown function. As the three-dimensional structure of an enzyme is a prerequisite for the rational development of novel inhibitors, we present here the crystal structure of the SIS domain of a catalytic mutant (K59A) of E. coli D-arabinose-5-phosphate isomerase at 2.6-A resolution. Our structural analyses and comparisons made with other SIS domains highlight several potentially important active site residues. In particular, the crystal structure allowed us to identify a previously unpredicted His residue (H88) located at the mouth of the active site cavity as a possible catalytic residue. On the basis of such structural data, subsequently supported by biochemical and mutational experiments, we confirm the catalytic role of H88, which appears to be a generally conserved residue among two-domain isomerases. | |||
Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography.,Gourlay LJ, Sommaruga S, Nardini M, Sperandeo P, Deho G, Polissi A, Bolognesi M Protein Sci. 2010 Dec;19(12):2430-9. doi: 10.1002/pro.525. PMID:20954237<ref>PMID:20954237</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Arabinose-5-phosphate isomerase]] | [[Category: Arabinose-5-phosphate isomerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Bolognesi, M | [[Category: Bolognesi, M]] | ||
[[Category: Deho, G | [[Category: Deho, G]] | ||
[[Category: Gourlay, L J | [[Category: Gourlay, L J]] | ||
[[Category: Nardini, M | [[Category: Nardini, M]] | ||
[[Category: Polissi, A | [[Category: Polissi, A]] | ||
[[Category: Sommaruga, S | [[Category: Sommaruga, S]] | ||
[[Category: Sperandeo, P | [[Category: Sperandeo, P]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
[[Category: Lipopolysaccharide biogenesis]] | [[Category: Lipopolysaccharide biogenesis]] | ||
