3fp5: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "3fp5" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image: | ==Crystal structure of ACBP from Moniliophthora perniciosa== | ||
<StructureSection load='3fp5' size='340' side='right' caption='[[3fp5]], [[Resolution|resolution]] 1.61Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3fp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Moniliophthora_perniciosa Moniliophthora perniciosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FP5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MpACBP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=153609 Moniliophthora perniciosa])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3fp5 PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fp5_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Acyl-CoA binding protein (ACBP) is a housekeeping protein and is an essential protein in human cell lines and in Trypanosoma brucei. The ACBP of Moniliophthora perniciosa is composed of 104 amino acids and is possibly a non-classic isoform exclusively from Basidiomycetes. The M. perniciosa acbp gene was cloned, and the protein was expressed and purified. Acyl-CoA ester binding was analyzed by isoelectric focusing, native gel electrophoresis and isothermal titration calorimetry. Our results suggest an increasing affinity of ACBP for longer acyl-CoA esters, such as myristoyl-CoA to arachidoyl-CoA, and best fit modeling indicates two binding sites. ACBP undergoes a shift from a monomeric to a dimeric state, as shown by dynamic light scattering, fluorescence anisotropy and native gel electrophoresis in the absence and presence of the ligand. The protein's structure was determined at 1.6 A resolution and revealed a new topology for ACBP, containing five alpha-helices instead of four. alpha-helices 1, 2, 3 and 4 adopted a bundled arrangement that is unique from the previously determined four-helix folds of ACBP, while alpha-helices 1, 2, 4 and 5 formed a classical four-helix bundle. A MES molecule was found in the CoA binding site, suggesting that the CoA site could be a target for small compound screening. | |||
A new topology of ACBP from Moniliophthora perniciosa.,Monzani PS, Pereira HM, Melo FA, Meirelles FV, Oliva G, Cascardo JC Biochim Biophys Acta. 2010 Jan;1804(1):115-23. Epub 2009 Sep 24. PMID:19782157<ref>PMID:19782157</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Moniliophthora perniciosa]] | [[Category: Moniliophthora perniciosa]] | ||
[[Category: Cascardo, J C.M | [[Category: Cascardo, J C.M]] | ||
[[Category: Meirelles, F V | [[Category: Meirelles, F V]] | ||
[[Category: Melo, F A | [[Category: Melo, F A]] | ||
[[Category: Monzani, P S | [[Category: Monzani, P S]] | ||
[[Category: Oliva, G | [[Category: Oliva, G]] | ||
[[Category: Pereira, H M | [[Category: Pereira, H M]] | ||
[[Category: Acbp]] | [[Category: Acbp]] | ||
[[Category: Cacao disease]] | [[Category: Cacao disease]] | ||
[[Category: Fatty acid metabolism]] | [[Category: Fatty acid metabolism]] | ||
[[Category: Lipid binding protein]] | [[Category: Lipid binding protein]] | ||
Revision as of 10:08, 3 December 2014
Crystal structure of ACBP from Moniliophthora perniciosa
| ||||||||||||
