3fxb: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "3fxb" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image: | ==Crystal structure of the ectoine-binding protein UehA== | ||
<StructureSection load='3fxb' size='340' side='right' caption='[[3fxb]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3fxb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruegeria_pomeroyi_dss-3 Ruegeria pomeroyi dss-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FXB FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4CS:(4S)-2-METHYL-1,4,5,6-TETRAHYDROPYRIMIDINE-4-CARBOXYLIC+ACID'>4CS</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPO1147 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246200 Ruegeria pomeroyi DSS-3])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fxb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fxb RCSB], [http://www.ebi.ac.uk/pdbsum/3fxb PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/3fxb_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Substrate-binding proteins or extracellular solute receptors (ESRs) are components of both ABC (ATP binding cassette) and TRAP-T (tripartite ATP-independent periplasmic transporter). The TRAP-T system UehABC from Silicibacter pomeroyi DSS-3 imports the compatible solutes ectoine and 5-hydroxyectoine as nutrients. UehA, the ESR of the UehABC operon, binds both ectoine and 5-hydroxyectoine with high affinity (K(d) values of 1.4+/-0.1 and 1.1+/-0.1 microM, respectively) and delivers them to the TRAP-T complex. The crystal structure of UehA in complex with ectoine was determined at 2.9-A resolution and revealed an overall fold common for all ESR proteins from TRAP systems determined so far. A comparison of the recently described structure of TeaA from Halomonas elongata and an ectoine-binding protein (EhuB) from an ABC transporter revealed a conserved ligand binding mode that involves both directed and cation-pi interactions. Furthermore, a comparison with other known TRAP-T ESRs revealed a helix that might act as a selectivity filter imposing restraints on the ESRs that fine-tune ligand recognition and binding and finally might determine the selection of the cognate substrate. | |||
The crystal structure of UehA in complex with ectoine-A comparison with other TRAP-T binding proteins.,Lecher J, Pittelkow M, Zobel S, Bursy J, Bonig T, Smits SH, Schmitt L, Bremer E J Mol Biol. 2009 May 29;389(1):58-73. Epub 2009 Apr 10. PMID:19362561<ref>PMID:19362561</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Ruegeria pomeroyi dss-3]] | [[Category: Ruegeria pomeroyi dss-3]] | ||
[[Category: Bremer, E | [[Category: Bremer, E]] | ||
[[Category: Bursy, J | [[Category: Bursy, J]] | ||
[[Category: Lecher, J | [[Category: Lecher, J]] | ||
[[Category: Pittelkow, M | [[Category: Pittelkow, M]] | ||
[[Category: Schmitt, L | [[Category: Schmitt, L]] | ||
[[Category: Smits, S H.J | [[Category: Smits, S H.J]] | ||
[[Category: Membrane]] | [[Category: Membrane]] | ||
[[Category: Periplasmic substrate binding protein]] | [[Category: Periplasmic substrate binding protein]] | ||
