2abw: Difference between revisions
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[[Image:2abw.gif|left|200px]] | [[Image:2abw.gif|left|200px]] | ||
'''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)''' | {{Structure | ||
|PDB= 2abw |SIZE=350|CAPTION= <scene name='initialview01'>2abw</scene>, resolution 1.620Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene> | |||
|ACTIVITY= | |||
|GENE= Pdx2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | |||
}} | |||
'''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2ABW is a [ | 2ABW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABW OCA]. | ||
==Reference== | ==Reference== | ||
Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:[http:// | Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16339145 16339145] | ||
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vitamin b6]] | [[Category: vitamin b6]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:47:49 2008'' | ||
Revision as of 13:47, 20 March 2008
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| 2abw, resolution 1.620Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | PG4 | ||||||||||||
| Gene: | Pdx2 (Plasmodium falciparum) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)
Overview
Vitamin B6 is one of nature's most versatile cofactors. Most organisms synthesize vitamin B6 via a recently discovered pathway employing the proteins Pdx1 and Pdx2. Here we present an in-depth characterization of the respective orthologs from the malaria parasite, Plasmodium falciparum. Expression profiling of Pdx1 and -2 shows that blood-stage parasites indeed possess a functional vitamin B6 de novo biosynthesis. Recombinant Pdx1 and Pdx2 form a complex that functions as a glutamine amidotransferase with Pdx2 as the glutaminase and Pdx1 as pyridoxal-5 '-phosphate synthase domain. Complex formation is required for catalytic activity of either domain. Pdx1 forms a chimeric bi-enzyme with the bacterial YaaE, a Pdx2 ortholog, both in vivo and in vitro, although this chimera does not attain full catalytic activity, emphasizing that species-specific structural features govern the interaction between the protein partners of the PLP synthase complexes in different organisms. To gain insight into the activation mechanism of the parasite bi-enzyme complex, the three-dimensional structure of Pdx2 was determined at 1.62 A. The obstruction of the oxyanion hole indicates that Pdx2 is in a resting state and that activation occurs upon Pdx1-Pdx2 complex formation.
About this Structure
2ABW is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:16339145
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