4fgn: Difference between revisions
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[[ | ==Crystal structure of the SV40 large T-antigen origin bining domain bound to Site I DNA== | ||
<StructureSection load='4fgn' size='340' side='right' caption='[[4fgn]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4fgn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FGN FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fuf|2fuf]], [[2ntc|2ntc]], [[2itl|2itl]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fgn RCSB], [http://www.ebi.ac.uk/pdbsum/4fgn PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Polyomavirus origins of replication contain multiple G(A/G)GGC sequences; the high affinity binding element for the viral initiator T-antigen (T-ag). The Site I regulatory region of Simian Virus 40, involved in the repression of transcription and the enhancement of DNA replication initiation, contains two GAGGC sequences arranged head-to-tail and separated by a 7 bp AT-rich sequence. We have solved a 3.2 A co-structure of the SV40 origin-binding domain (OBD) bound to Site I. We have also established that T-ag assembly on Site I is limited to the formation of a single hexamer. These observations have enabled an analyses of the role(s) of the OBDs bound to the Site I pentanucleotides in hexamer formation. Of interest, they reveal a correlation between the OBDs bound to Site I and a pair of OBD subunits in the previously described hexameric spiral structure. Based on these findings, we propose that spiral assembly is promoted by pentanucleotide pairs arranged in a head to tail manner. Finally, the possibility that "spiral-assembly " by OBD subunits may account for the heterogeneous distribution of pentanucleotides found in the origins of replication of polyomaviruses is discussed. | |||
ANALYSIS OF THE CO-STRUCTURE OF THE SV40 T-ANTIGEN ORIGIN BINDING DOMAIN WITH SITE I REVEALS A CORRELATION BETWEEN GAGGC SPACING AND SPIRAL ASSEMBLY.,Meinke G, Phelan PJ, Harrison C, Bullock PA J Virol. 2012 Dec 26. PMID:23269808<ref>PMID:23269808</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Large T Antigen|Large T Antigen]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
[[Category: Bohm, A | [[Category: Bohm, A]] | ||
[[Category: Bullock, P A | [[Category: Bullock, P A]] | ||
[[Category: Meinke, G | [[Category: Meinke, G]] | ||
[[Category: Dna binding protein-dna complex]] | [[Category: Dna binding protein-dna complex]] | ||
[[Category: Origin binding domain]] | [[Category: Origin binding domain]] | ||
