2ahq: Difference between revisions
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[[Image:2ahq.gif|left|200px]] | [[Image:2ahq.gif|left|200px]] | ||
'''Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus''' | {{Structure | ||
|PDB= 2ahq |SIZE=350|CAPTION= <scene name='initialview01'>2ahq</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= rpoN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |||
}} | |||
'''Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AHQ is a [ | 2AHQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHQ OCA]. | ||
==Reference== | ==Reference== | ||
The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70., Doucleff M, Malak LT, Pelton JG, Wemmer DE, J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:[http:// | The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70., Doucleff M, Malak LT, Pelton JG, Wemmer DE, J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16210314 16210314] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Wemmer, D E.]] | [[Category: Wemmer, D E.]] | ||
[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
[[Category: sigma | [[Category: sigma factor]] | ||
[[Category: sigma-54]] | [[Category: sigma-54]] | ||
[[Category: solution structure]] | [[Category: solution structure]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:49:53 2008'' | ||
Revision as of 13:49, 20 March 2008
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| Gene: | rpoN (Aquifex aeolicus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus
Overview
The "sigma" subunit of prokaryotic RNA polymerase allows gene-specific transcription initiation. Two sigma families have been identified, sigma70 and sigma54, which use distinct mechanisms to initiate transcription and share no detectable sequence homology. Although the sigma70-type factors have been well characterized structurally by x-ray crystallography, no high resolution structural information is available for the sigma54-type factors. Here we present the NMR-derived structure of the C-terminal domain of sigma54 from Aquifex aeolicus. This domain (Thr-323 to Gly-389), which contains the highly conserved RpoN box sequence, consists of a poorly structured N-terminal tail followed by a three-helix bundle, which is surprisingly similar to domains of the sigma70-type proteins. Residues of the RpoN box, which have previously been shown to be critical for DNA binding, form the second helix of an unpredicted helix-turn-helix motif. The homology of this structure with other DNA-binding proteins, combined with previous biochemical data, suggests how the C-terminal domain of sigma54 binds to DNA.
About this Structure
2AHQ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70., Doucleff M, Malak LT, Pelton JG, Wemmer DE, J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:16210314
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