2avw: Difference between revisions
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[[Image:2avw.gif|left|200px]] | [[Image:2avw.gif|left|200px]] | ||
'''Crystal structure of monoclinic form of streptococcus Mac-1''' | {{Structure | ||
|PDB= 2avw |SIZE=350|CAPTION= <scene name='initialview01'>2avw</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= mac ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198466 Streptococcus pyogenes MGAS315]) | |||
}} | |||
'''Crystal structure of monoclinic form of streptococcus Mac-1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AVW is a [ | 2AVW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas315 Streptococcus pyogenes mgas315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http:// | Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16472742 16472742] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus pyogenes mgas315]] | [[Category: Streptococcus pyogenes mgas315]] | ||
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[[Category: mac-1]] | [[Category: mac-1]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:54:29 2008'' | ||
Revision as of 13:54, 20 March 2008
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| 2avw, resolution 2.0Å | |||||||||||||
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| Ligands: | SO4 and GOL | ||||||||||||
| Gene: | mac (Streptococcus pyogenes MGAS315) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of monoclinic form of streptococcus Mac-1
Overview
Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2 that mediate host immune evasion by targeting both IgG and Fc receptors. Here, we report the crystal structures of Mac-1 and its catalytically inactive C94A mutant in two different crystal forms. Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold. Alanine mutations at the active site confirmed the critical residues involved in a papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both crystal forms and displays the unique dimer interface among papain superfamily members. Mutations at the dimer interface resulted in a significant reduction in IgG binding and catalysis, suggesting that the dimer contributes to both IgG specificity and enzyme cooperativity. A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2.
About this Structure
2AVW is a Single protein structure of sequence from Streptococcus pyogenes mgas315. Full crystallographic information is available from OCA.
Reference
Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:16472742
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