4h2t: Difference between revisions
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{{STRUCTURE_4h2t| PDB=4h2t | SCENE= }} | {{STRUCTURE_4h2t| PDB=4h2t | SCENE= }} | ||
===Crystal structure of Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with cognate carrier protein and an analogue of glycyl adenylate=== | ===Crystal structure of Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with cognate carrier protein and an analogue of glycyl adenylate=== | ||
{{ABSTRACT_PUBMED_23541895}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/AACL1_BRAJA AACL1_BRAJA]] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [[http://www.uniprot.org/uniprot/AACP1_BRAJA AACP1_BRAJA]] Aminoacyl carrier protein. Can be charged with L-glycine via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the bll0957 ligase. | |||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
<ref group="xtra">PMID:020663952</ref><references group="xtra"/> | <ref group="xtra">PMID:020663952</ref><references group="xtra"/><references/> | ||
[[Category: Bradyrhizobium japonicum usda 110]] | [[Category: Bradyrhizobium japonicum usda 110]] | ||
[[Category: Ivic, N.]] | [[Category: Ivic, N.]] | ||
Revision as of 21:45, 10 April 2013
Crystal structure of Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with cognate carrier protein and an analogue of glycyl adenylate
Template:ABSTRACT PUBMED 23541895
Function
[AACL1_BRAJA] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [AACP1_BRAJA] Aminoacyl carrier protein. Can be charged with L-glycine via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the bll0957 ligase.
About this Structure
4h2t is a 4 chain structure with sequence from Bradyrhizobium japonicum usda 110. Full crystallographic information is available from OCA.
Reference
- Mocibob M, Ivic N, Bilokapic S, Maier T, Luic M, Ban N, Weygand-Durasevic I. Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis. Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14585-90. Epub 2010 Jul 27. PMID:20663952 doi:10.1073/pnas.1007470107
