3hgd: Difference between revisions
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[[ | ==Crystal Structure of 2-Se-Thymidine Derivatized DNA== | ||
<StructureSection load='3hgd' size='340' side='right' caption='[[3hgd]], [[Resolution|resolution]] 1.57Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3hgd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HGD FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene>, <scene name='pdbligand=US3:1-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-5-METHYL-2-SELANYLPYRIMIDIN-4(1H)-ONE'>US3</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hg8|3hg8]], [[3hga|3hga]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hgd RCSB], [http://www.ebi.ac.uk/pdbsum/3hgd PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The base pairs are the contributors to the sequence-dependent recognition of nucleic acids, genetic information storage, and high fidelity of DNA polymerase replication. However, the wobble base pairing, where T pairs with G instead of A, reduces specific base-pairing recognition and compromises the high fidelity of the enzymatic polymerization. Via the selenium atomic probing at the 2-position of thymidine, we have investigated the wobble discrimination by manipulating the steric and electronic effects at the 2-exo position, providing a unique chemical strategy to enhance the base pair specificity. We report here the first synthesis of the novel 2-Se-thymidine ((Se)T) derivative, its phosphoramidite, and the Se-DNAs. Our biophysical and structural studies of the 2-Se-T DNAs reveal that the bulky 2-Se atom with a weak hydrogen-bonding ability can largely increase mismatch discriminations (including T/G wobble and T/C mismatched base pairs) while maintaining the (Se)T/A virtually identical to the native T/A base pair. The 2-Se atom bulkiness and the electronic effect are probably the main factors responsible for the discrimination against the formation of the wobble (Se)T/G base pair. Our investigations provide a potential novel tool to investigate the specific recognition of base pairs, which is the basis of high fidelity during replication, transcription, and translation. Furthermore, this Se-atom-specific substitution and probing are useful for X-ray crystal structure and function studies of nucleic acids. | |||
High Fidelity of Base Pairing by 2-Selenothymidine in DNA.,Hassan AE, Sheng J, Zhang W, Huang Z J Am Chem Soc. 2010 Jan 28. PMID:20108896<ref>PMID:20108896</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hassan, A E]] | |||
== | [[Category: Huang, Z]] | ||
< | [[Category: Sheng, J]] | ||
[[Category: Hassan, A E | [[Category: Zhang, W]] | ||
[[Category: Huang, Z | |||
[[Category: Sheng, J | |||
[[Category: Zhang, W | |||
[[Category: 2-se-thymidine dna]] | [[Category: 2-se-thymidine dna]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
Revision as of 08:47, 8 December 2014
Crystal Structure of 2-Se-Thymidine Derivatized DNA
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