2b2s: Difference between revisions
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[[Image:2b2s.gif|left|200px]] | [[Image:2b2s.gif|left|200px]] | ||
'''Crystal structure of an oxoferryl species of catalase-peroxidase KATG at pH7.5''' | {{Structure | ||
|PDB= 2b2s |SIZE=350|CAPTION= <scene name='initialview01'>2b2s</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=O:OXYGEN ATOM'>O</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] | |||
|GENE= katG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei]) | |||
}} | |||
'''Crystal structure of an oxoferryl species of catalase-peroxidase KATG at pH7.5''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2B2S is a [ | 2B2S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2S OCA]. | ||
==Reference== | ==Reference== | ||
A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases., Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, Fita I, Loewen PC, EMBO Rep. 2005 Dec;6(12):1156-62. PMID:[http:// | A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases., Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, Fita I, Loewen PC, EMBO Rep. 2005 Dec;6(12):1156-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16211084 16211084] | ||
[[Category: Burkholderia pseudomallei]] | [[Category: Burkholderia pseudomallei]] | ||
[[Category: Catalase]] | [[Category: Catalase]] | ||
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[[Category: oxoferryl species]] | [[Category: oxoferryl species]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:56:54 2008'' | ||
Revision as of 13:56, 20 March 2008
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| 2b2s, resolution 2.00Å | |||||||||||||
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| Ligands: | NA, HEM and O | ||||||||||||
| Gene: | katG (Burkholderia pseudomallei) | ||||||||||||
| Activity: | Catalase, with EC number 1.11.1.6 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of an oxoferryl species of catalase-peroxidase KATG at pH7.5
Overview
The catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. An arginine, 20 A from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by H(2)O(2), facilitating the catalatic pathway in a peroxidase. The influence of the arginine is imparted to the heme through its association with or dissociation from a tyrosinate that modulates reactivity through a Met-Tyr-Trp crosslinked adduct and a pi electron interaction of the heme with the adduct Trp.
About this Structure
2B2S is a Single protein structure of sequence from Burkholderia pseudomallei. Full crystallographic information is available from OCA.
Reference
A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases., Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, Fita I, Loewen PC, EMBO Rep. 2005 Dec;6(12):1156-62. PMID:16211084
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