2efw: Difference between revisions
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==Crystal structure of the RTP:nRB complex from Bacillus subtilis== | |||
=== | <StructureSection load='2efw' size='340' side='right' caption='[[2efw]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2efw]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EFW FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bm9|1bm9]], [[1j0r|1j0r]], [[1f4k|1f4k]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rtp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2efw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2efw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2efw RCSB], [http://www.ebi.ac.uk/pdbsum/2efw PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In Bacillus subtilis, the termination of DNA replication via polar fork arrest is effected by a specific protein:DNA complex formed between the replication terminator protein (RTP) and DNA terminator sites. We report the crystal structure of a replication terminator protein homologue (RTP.C110S) of B. subtilis in complex with the high affinity component of one of its cognate DNA termination sites, known as the TerI B-site, refined at 2.5 A resolution. The 21 bp RTP:DNA complex displays marked structural asymmetry in both the homodimeric protein and the DNA. This is in contrast to the previously reported complex formed with a symmetrical TerI B-site homologue. The induced asymmetry is consistent with the complex's solution properties as determined using NMR spectroscopy. Concomitant with this asymmetry is variation in the protein:DNA binding pattern for each of the subunits of the RTP homodimer. It is proposed that the asymmetric "wing" positions, as well as other asymmetrical features of the RTP:DNA complex, are critical for the cooperative binding that underlies the mechanism of polar fork arrest at the complete terminator site. | |||
An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA.,Vivian JP, Porter CJ, Wilce JA, Wilce MC J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:17521668<ref>PMID:17521668</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Replication Termination Protein|Replication Termination Protein]] | *[[Replication Termination Protein|Replication Termination Protein]] | ||
*[[User:Chloe Paul/Replication Terminator Protein|User:Chloe Paul/Replication Terminator Protein]] | *[[User:Chloe Paul/Replication Terminator Protein|User:Chloe Paul/Replication Terminator Protein]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Porter, C J | [[Category: Porter, C J]] | ||
[[Category: Vivian, J P | [[Category: Vivian, J P]] | ||
[[Category: Wilce, J A | [[Category: Wilce, J A]] | ||
[[Category: Wilce, M C.J | [[Category: Wilce, M C.J]] | ||
[[Category: Winged -helix protein]] | [[Category: Winged -helix protein]] | ||
[[Category: Dna replication termination]] | [[Category: Dna replication termination]] | ||
