4ab1: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Recombinant Human Carboxylesterase 1 from whole Cabbage Loopers== | |||
=== | <StructureSection load='4ab1' size='340' side='right' caption='[[4ab1]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ab1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AB1 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mx1|1mx1]], [[1mx5|1mx5]], [[1mx9|1mx9]], [[1ya4|1ya4]], [[1ya8|1ya8]], [[1yah|1yah]], [[1yaj|1yaj]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ab1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ab1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ab1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ab1 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The use of whole insect larvae as a source of recombinant proteins offers a more cost-effective method of producing large quantities of human proteins than conventional cell-culture approaches. Human carboxylesterase 1 has been produced in and isolated from whole Trichoplusia ni larvae. The recombinant protein was crystallized and its structure was solved to 2.2 resolution. The results indicate that the larvae-produced enzyme is essentially identical to that isolated from cultured Sf21 cells, supporting the use of this expression system to produce recombinant enzymes for crystallization studies. | |||
Structure of recombinant human carboxylesterase 1 isolated from whole cabbage looper larvae.,Greenblatt HM, Otto TC, Kirkpatrick MG, Kovaleva E, Brown S, Buchman G, Cerasoli DM, Sussman JL Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):269-72., Epub 2012 Feb 15. PMID:22442219<ref>PMID:22442219</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Carboxylesterase|Carboxylesterase]] | *[[Carboxylesterase|Carboxylesterase]] | ||
*[[Journal:Acta Cryst F:1|Journal:Acta Cryst F:1]] | *[[Journal:Acta Cryst F:1|Journal:Acta Cryst F:1]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Cerasoli, D M | [[Category: Cerasoli, D M]] | ||
[[Category: Greenblatt, H M | [[Category: Greenblatt, H M]] | ||
[[Category: Otto, T C | [[Category: Otto, T C]] | ||
[[Category: Sussman, J L | [[Category: Sussman, J L]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Whole insect expression system]] | [[Category: Whole insect expression system]] | ||
Revision as of 08:36, 18 December 2014
Recombinant Human Carboxylesterase 1 from whole Cabbage Loopers
| ||||||||||||
