2bac: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bac.gif|left|200px]] | [[Image:2bac.gif|left|200px]] | ||
'''Crystal structure of CLA-producing fatty acid isomerase from P. acnes''' | {{Structure | ||
|PDB= 2bac |SIZE=350|CAPTION= <scene name='initialview01'>2bac</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=ODT:(11E,13E,15Z)-OCTADECA-11,13,15-TRIENOIC ACID'>ODT</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of CLA-producing fatty acid isomerase from P. acnes''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2BAC is a [ | 2BAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Propionibacterium_acnes Propionibacterium acnes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAC OCA]. | ||
==Reference== | ==Reference== | ||
Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase., Liavonchanka A, Hornung E, Feussner I, Rudolph MG, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2576-81. Epub 2006 Feb 13. PMID:[http:// | Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase., Liavonchanka A, Hornung E, Feussner I, Rudolph MG, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2576-81. Epub 2006 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16477020 16477020] | ||
[[Category: Propionibacterium acnes]] | [[Category: Propionibacterium acnes]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: isomerase]] | [[Category: isomerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:59:24 2008'' | ||
Revision as of 13:59, 20 March 2008
| |||||||||||||
| 2bac, resolution 2.30Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | SO4, FAD and ODT | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of CLA-producing fatty acid isomerase from P. acnes
Overview
Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of CLA exist, of which the (9Z, 11E) and (10E, 12Z) isomers have beneficial effects on human metabolism but are scarce in foods. Bacterial polyunsaturated fatty acid isomerases are promising biotechnological catalysts for CLA production. We describe six crystal structures of the Propionibacterium acnes polyunsaturated fatty acid isomerase PAI in apo- and product-bound forms. The three-domain flavoprotein has previously undescribed folds outside the FAD-binding site. Conformational changes in a hydrophobic channel toward the active site reveal a unique gating mechanism for substrate specificity. The geometry of the substrate-binding site explains the length preferences for C18 fatty acids. A catalytic mechanism for double-bond isomerization is formulated that may be altered to change substrate specificity for syntheses of rare CLAs from easily accessible precursors.
About this Structure
2BAC is a Single protein structure of sequence from Propionibacterium acnes. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase., Liavonchanka A, Hornung E, Feussner I, Rudolph MG, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2576-81. Epub 2006 Feb 13. PMID:16477020
Page seeded by OCA on Thu Mar 20 15:59:24 2008
