1o7c: Difference between revisions
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==Overview== | ==Overview== | ||
The solution structure of the Link module from human TSG-6, a hyaladherin, with important roles in inflammation and ovulation, has been determined in, both its free and hyaluronan-bound conformations. This reveals a well, defined hyaluronan-binding groove on one face of the Link module that is, closed in the absence of ligand. The groove is lined with amino acids that, have been implicated in mediating the interaction with hyaluronan, including two tyrosine residues that appear to form essential, intermolecular hydrogen bonds and two basic residues capable of supporting, ionic interactions. This is the first structure of a non-enzymic, hyaladherin in its active state, and identifies a ligand-induced, conformational change that is likely to be conserved across the Link, module superfamily. . | The solution structure of the Link module from human TSG-6, a hyaladherin, with important roles in inflammation and ovulation, has been determined in, both its free and hyaluronan-bound conformations. This reveals a well, defined hyaluronan-binding groove on one face of the Link module that is, closed in the absence of ligand. The groove is lined with amino acids that, have been implicated in mediating the interaction with hyaluronan, including two tyrosine residues that appear to form essential, intermolecular hydrogen bonds and two basic residues capable of supporting, ionic interactions. This is the first structure of a non-enzymic, hyaladherin in its active state, and identifies a ligand-induced, conformational change that is likely to be conserved across the Link, module superfamily. NMR and isothermal titration calorimetry experiments, with defined oligosaccharides have allowed us to infer the minimum length, of hyaluronan that can be accommodated within the binding site and its, polarity in the groove; these data have been used to generate a model of, the complex formed between the Link module and a hyaluronan, octasaccharide. | ||
==About this Structure== | ==About this Structure== | ||
1O7C is a | 1O7C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Structure known Active Site: HYA. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O7C OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: link module]] | [[Category: link module]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:11:59 2007'' | ||
