2kqt: Difference between revisions
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==Solid-state NMR structure of the M2 transmembrane peptide of the influenza A virus in DMPC lipid bilayers bound to deuterated amantadine== | |||
<StructureSection load='2kqt' size='340' side='right' caption='[[2kqt]], [[NMR_Ensembles_of_Models | 17 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kqt]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KQT FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=308:(3S,5S,7S)-TRICYCLO[3.3.1.1~3,7~]DECAN-1-AMINE'>308</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kad|2kad]], [[2h95|2h95]], [[2rlf|2rlf]], [[3c9j|3c9j]], [[1nyj|1nyj]], [[3bkd|3bkd]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kqt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kqt RCSB], [http://www.ebi.ac.uk/pdbsum/2kqt PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine. | |||
Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus.,Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:17384070<ref>PMID:17384070</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
| Line 10: | Line 20: | ||
*[[M2 protein|M2 protein]] | *[[M2 protein|M2 protein]] | ||
*[[Molecular Playground/Influenza A M2 transmembrane domain|Molecular Playground/Influenza A M2 transmembrane domain]] | *[[Molecular Playground/Influenza A M2 transmembrane domain|Molecular Playground/Influenza A M2 transmembrane domain]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
[[Category: Cady, S D | </StructureSection> | ||
[[Category: DeGrado, W F | [[Category: Cady, S D]] | ||
[[Category: Hong, M | [[Category: DeGrado, W F]] | ||
[[Category: Schmidt-Rohr, K | [[Category: Hong, M]] | ||
[[Category: Soto, C S | [[Category: Schmidt-Rohr, K]] | ||
[[Category: Wang, J | [[Category: Soto, C S]] | ||
[[Category: Wang, J]] | |||
[[Category: Amantadine]] | [[Category: Amantadine]] | ||
[[Category: Influenza]] | [[Category: Influenza]] | ||
Revision as of 14:03, 17 December 2014
Solid-state NMR structure of the M2 transmembrane peptide of the influenza A virus in DMPC lipid bilayers bound to deuterated amantadine
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