2bdz: Difference between revisions
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'''Mexicain from Jacaratia mexicana''' | {{Structure | ||
|PDB= 2bdz |SIZE=350|CAPTION= <scene name='initialview01'>2bdz</scene>, resolution 2.100Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Mexicain from Jacaratia mexicana''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2BDZ is a [ | 2BDZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA]. | ||
==Reference== | ==Reference== | ||
Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:[http:// | Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502326 15502326] | ||
[[Category: Jacaratia mexicana]] | [[Category: Jacaratia mexicana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: peptidase_c1]] | [[Category: peptidase_c1]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:00:39 2008'' | ||
Revision as of 14:00, 20 March 2008
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| 2bdz, resolution 2.100Å | |||||||||||||
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| Ligands: | E64 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Mexicain from Jacaratia mexicana
Overview
Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.
About this Structure
2BDZ is a Single protein structure of sequence from Jacaratia mexicana. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326
Page seeded by OCA on Thu Mar 20 16:00:39 2008
