3ifi: Difference between revisions
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==2'-SeMe-dG modified octamer DNA== | |||
===2'-SeMe- | <StructureSection load='3ifi' size='340' side='right' caption='[[3ifi]], [[Resolution|resolution]] 1.20Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ifi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IFI FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XUG:2-SE-METHYL-2-SELENOGUANOSINE+5-(DIHYDROGEN+PHOSPHATE)'>XUG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dns|1dns]], [[3iff|3iff]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ifi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ifi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ifi RCSB], [http://www.ebi.ac.uk/pdbsum/3ifi PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Selenium modification of nucleic acids is of great importance in X-ray crystal structure determination and functional study of nucleic acids. Herein, we describe a convenient synthesis of a new building block, the 2'-SeMe-modified guanosine (G(Se)) phosphoramidite, and report the first incorporation of the 2'-Se-G moiety into DNA. The X-ray crystal structure of the 2'-Se-modified octamer DNA (5'-GTG(Se)TACAC-3') was determined at a resolution of 1.20 A. We also found that the 2'-Se modification points to the minor groove and that the modified and native structures are virtually identical. Furthermore, we observed that the 2'-Se-G modification can significantly facilitate the crystal growth with respect to the corresponding native DNA. | |||
Synthesis and crystal structure of 2'-se-modified guanosine containing DNA.,Salon J, Sheng J, Gan J, Huang Z J Org Chem. 2010 Feb 5;75(3):637-41. PMID:20047333<ref>PMID:20047333</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
< | </div> | ||
[[Category: Gan, J | == References == | ||
[[Category: Huang, Z | <references/> | ||
[[Category: Salon, J | __TOC__ | ||
[[Category: Sheng, J | </StructureSection> | ||
[[Category: Gan, J]] | |||
[[Category: Huang, Z]] | |||
[[Category: Salon, J]] | |||
[[Category: Sheng, J]] | |||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Guanosine]] | [[Category: Guanosine]] | ||
[[Category: Nucleic acid]] | [[Category: Nucleic acid]] | ||
[[Category: Selenium]] | [[Category: Selenium]] | ||
