1oa0: Difference between revisions
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==Overview== | ==Overview== | ||
The hybrid cluster proteins from the sulfate reducing bacteria, Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris, strain Hildenborough ( Dv) have been isolated and crystallized, anaerobically. In each case, the protein has been reduced with dithionite, and the crystal structure of the reduced form elucidated using X-ray, synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd, and Dv, respectively. Although the overall structures of the proteins are, unchanged upon reduction, there are significant changes at the hybrid, cluster centres. These include significant movements in the position of, the iron atom linked to the persulfide moiety in the oxidized as-isolated, proteins and the sulfur atom of the persulfide itself. The nature of these, changes is . | The hybrid cluster proteins from the sulfate reducing bacteria, Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris, strain Hildenborough ( Dv) have been isolated and crystallized, anaerobically. In each case, the protein has been reduced with dithionite, and the crystal structure of the reduced form elucidated using X-ray, synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd, and Dv, respectively. Although the overall structures of the proteins are, unchanged upon reduction, there are significant changes at the hybrid, cluster centres. These include significant movements in the position of, the iron atom linked to the persulfide moiety in the oxidized as-isolated, proteins and the sulfur atom of the persulfide itself. The nature of these, changes is described and the implications with respect to the function of, hybrid cluster proteins are discussed. | ||
==About this Structure== | ==About this Structure== | ||
1OA0 is a | 1OA0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with SF4, SF3 and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OA0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: reduced forms]] | [[Category: reduced forms]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:18:45 2007'' | ||
