2crx: Difference between revisions
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[[Image:2crx.gif|left|200px]] | [[Image:2crx.gif|left|200px]] | ||
'''STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION''' | {{Structure | ||
|PDB= 2crx |SIZE=350|CAPTION= <scene name='initialview01'>2crx</scene>, resolution 2.5Å | |||
|SITE= <scene name='pdbsite=CIA:Recombinase+Active+Site'>CIA</scene> and <scene name='pdbsite=CIB:Recombinase+Active+Site'>CIB</scene> | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CRX is a [ | 2CRX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:[http:// | Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9670032 9670032] | ||
[[Category: Bacteriophage p1]] | [[Category: Bacteriophage p1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:19:12 2008'' | ||
Revision as of 14:19, 20 March 2008
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| 2crx, resolution 2.5Å | |||||||||||||
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| Sites: | CIA and CIB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION
Overview
We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.
About this Structure
2CRX is a Single protein structure of sequence from Bacteriophage p1. Full crystallographic information is available from OCA.
Reference
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032
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