1ogd: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
RbsD is the only protein whose biochemical function is unknown among the, six gene products of the rbs operon involved in the active transport of, ribose. FucU, a paralogue of RbsD conserved from bacteria to human, is, also the only protein whose function is unknown among the seven gene, products of the l-fucose regulon. Here we report the crystal structures of, Bacillus subtilis RbsD, which reveals a novel decameric toroidal assembly, of the protein. Nuclear magnetic resonance and other studies on RbsD, reveal that the intersubunit cleft of the protein binds specific forms of, d-ribose, but it does not have an enzyme activity toward the sugar., Likewise, FucU binds l-fucose but lacks an enzyme activity toward this, sugar. We conclude that RbsD and FucU are cytoplasmic sugar-binding, . | RbsD is the only protein whose biochemical function is unknown among the, six gene products of the rbs operon involved in the active transport of, ribose. FucU, a paralogue of RbsD conserved from bacteria to human, is, also the only protein whose function is unknown among the seven gene, products of the l-fucose regulon. Here we report the crystal structures of, Bacillus subtilis RbsD, which reveals a novel decameric toroidal assembly, of the protein. Nuclear magnetic resonance and other studies on RbsD, reveal that the intersubunit cleft of the protein binds specific forms of, d-ribose, but it does not have an enzyme activity toward the sugar., Likewise, FucU binds l-fucose but lacks an enzyme activity toward this, sugar. We conclude that RbsD and FucU are cytoplasmic sugar-binding, proteins, a novel class of proteins whose functional role may lie in, helping influx of the sugar substrates. | ||
==About this Structure== | ==About this Structure== | ||
1OGD is a | 1OGD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with RIP and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGD OCA]. | ||
==Reference== | ==Reference== | ||
| Line 22: | Line 22: | ||
[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:03:30 2007'' | ||
