1ogy: Difference between revisions
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==Overview== | ==Overview== | ||
The structure of the respiratory nitrate reductase (NapAB) from, Rhodobacter sphaeroides, the periplasmic heterodimeric enzyme responsible, for the first step in the denitrification process, has been determined at, a resolution of 3.2 A. The di-heme electron transfer small subunit NapB, binds to the large subunit with heme II in close proximity to the [4Fe-4S], cluster of NapA. A total of 57 residues at the N- and C-terminal, extremities of NapB adopt an extended conformation, embracing the NapA, subunit and largely contributing to the total area of 5,900 A(2) buried in, the complex. Complex formation was studied further by measuring the, variation of the redox potentials of all the cofactors upon binding. The, marked effects observed are interpreted in light of the three-dimensional, . | The structure of the respiratory nitrate reductase (NapAB) from, Rhodobacter sphaeroides, the periplasmic heterodimeric enzyme responsible, for the first step in the denitrification process, has been determined at, a resolution of 3.2 A. The di-heme electron transfer small subunit NapB, binds to the large subunit with heme II in close proximity to the [4Fe-4S], cluster of NapA. A total of 57 residues at the N- and C-terminal, extremities of NapB adopt an extended conformation, embracing the NapA, subunit and largely contributing to the total area of 5,900 A(2) buried in, the complex. Complex formation was studied further by measuring the, variation of the redox potentials of all the cofactors upon binding. The, marked effects observed are interpreted in light of the three-dimensional, structure and depict a plasticity that contributes to an efficient, electron transfer in the complex from the heme I of NapB to the molybdenum, catalytic site of NapA. | ||
==About this Structure== | ==About this Structure== | ||
1OGY is a | 1OGY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with SF4, MO, MGD and HEC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:04:10 2007'' | ||
