4eob: Difference between revisions
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== | ==Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa== | ||
[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <StructureSection load='4eob' size='340' side='right' caption='[[4eob]], [[Resolution|resolution]] 2.61Å' scene=''> | ||
[[ | == Structural highlights == | ||
[[Category: Chou, S | <table><tr><td colspan='2'>[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EOB FirstGlance]. <br> | ||
[[Category: Mougous, J D | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA1844 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eob OCA], [http://pdbe.org/4eob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eob RCSB], [http://www.ebi.ac.uk/pdbsum/4eob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eob ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gram-negative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity. | |||
Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.,Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD Cell Rep. 2012 Jun 28;1(6):656-64. Epub 2012 May 31. PMID:22813741<ref>PMID:22813741</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4eob" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pseae]] | |||
[[Category: Chou, S]] | |||
[[Category: Mougous, J D]] | |||
[[Category: Amidase]] | [[Category: Amidase]] | ||
[[Category: Bacteriolytic]] | [[Category: Bacteriolytic]] | ||
Revision as of 22:52, 4 August 2016
Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa
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