3zfw: Difference between revisions

Line 1:

~~{{STRUCTURE_3zfw| PDB=3zfw | SCENE= }}~~

==Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide==

===Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide===

~~{{ABSTRACT_PUBMED_23519214}}~~

== Structural highlights ==

<table><tr><td colspan='2'>[[3zfw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFW FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfw RCSB], [http://www.ebi.ac.uk/pdbsum/3zfw PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Kinesin-mediated cargo transport is required for many cellular functions and plays a key role in pathological processes. Structural information on how kinesins recognize their cargoes is required for a molecular understanding of this fundamental and ubiquitous process. Here, we present the crystal structure of the tetratricopeptide repeat of kinesin light chain 2 in complex with a cargo peptide harboring a 'tryptophan-acidic' motif derived from SKIP, a critical host determinant in Salmonella pathogenesis and a regulator of lysosomal positioning. Structural data together with biophysical, biochemical, and cellular assays allow us to propose a framework for intracellular transport based on the binding by kinesin-1 of W-acidic cargo motifs through a combination of electrostatic interactions and sequence-specific elements, providing direct molecular evidence of the mechanisms for kinesin-1:cargo recognition.

~~==Function==~~

Structural Basis For Kinesin-1:Cargo Recognition.,Pernigo S, Lamprecht A, Steiner RA, Dodding MP Science. 2013 Mar 21. PMID:23519214<ref>PMID:23519214</ref>

~~[[http~~:~~//www.uniprot.org/uniprot/PKHM2_HUMAN PKHM2_HUMAN]] May play a role in the regulation of conventional kinesin activity~~. ~~Required for maintenance of the Golgi apparatus organization~~. ~~May play a role in membrane tubulation~~.<ref>PMID:~~15905402~~</ref>

~~==About this Structure==~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[3zfw]] is~~ a ~~4 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens] and [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3ZFW OCA].~~

</div>

==~~Reference~~==

==See Also==

*[[Kinesin|Kinesin]]

== References ==

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Mus musculus]]

[[Category: Dodding, M P.]]

[[Category: Dodding, M P]]

[[Category: Lamprecht, A.]]

[[Category: Lamprecht, A]]

[[Category: Pernigo, S.]]

[[Category: Pernigo, S]]

[[Category: Steiner, R A.]]

[[Category: Steiner, R A]]

[[Category: Hydrolase]]

[[Category: Kinesin-cargo recognition]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_3zfw|  PDB=3zfw  |  SCENE=  }}
+==Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide==
-===Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide===
+<StructureSection load='3zfw' size='340' side='right' caption='[[3zfw]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23519214}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3zfw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFW FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfw RCSB], [http://www.ebi.ac.uk/pdbsum/3zfw PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Kinesin-mediated cargo transport is required for many cellular functions and plays a key role in pathological processes. Structural information on how kinesins recognize their cargoes is required for a molecular understanding of this fundamental and ubiquitous process. Here, we present the crystal structure of the tetratricopeptide repeat of kinesin light chain 2 in complex with a cargo peptide harboring a 'tryptophan-acidic' motif derived from SKIP, a critical host determinant in Salmonella pathogenesis and a regulator of lysosomal positioning. Structural data together with biophysical, biochemical, and cellular assays allow us to propose a framework for intracellular transport based on the binding by kinesin-1 of W-acidic cargo motifs through a combination of electrostatic interactions and sequence-specific elements, providing direct molecular evidence of the mechanisms for kinesin-1:cargo recognition.
-==Function==
+Structural Basis For Kinesin-1:Cargo Recognition.,Pernigo S, Lamprecht A, Steiner RA, Dodding MP Science. 2013 Mar 21. PMID:23519214<ref>PMID:23519214</ref>
-[[http://www.uniprot.org/uniprot/PKHM2_HUMAN PKHM2_HUMAN]] May play a role in the regulation of conventional kinesin activity. Required for maintenance of the Golgi apparatus organization. May play a role in membrane tubulation.<ref>PMID:15905402</ref>
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[3zfw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFW OCA].
+</div>
-==Reference==
+==See Also==
-<references group="xtra"/><references/>
+*[[Kinesin|Kinesin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
 [[Category: Mus musculus]]
-[[Category: Dodding, M P.]]
+[[Category: Dodding, M P]]
-[[Category: Lamprecht, A.]]
+[[Category: Lamprecht, A]]
-[[Category: Pernigo, S.]]
+[[Category: Pernigo, S]]
-[[Category: Steiner, R A.]]
+[[Category: Steiner, R A]]
 [[Category: Hydrolase]]
 [[Category: Kinesin-cargo recognition]]