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New page: left|200px<br /><applet load="2e9b" size="350" color="white" frame="true" align="right" spinBox="true" caption="2e9b, resolution 2.30Å" /> '''Crystal structure of...
 
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[[Image:2e9b.jpg|left|200px]]<br /><applet load="2e9b" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:2e9b.jpg|left|200px]]
caption="2e9b, resolution 2.30&Aring;" />
 
'''Crystal structure of pullulanase type I from Bacillus subtilis str. 168 complexed with maltose'''<br />
{{Structure
|PDB= 2e9b |SIZE=350|CAPTION= <scene name='initialview01'>2e9b</scene>, resolution 2.30&Aring;
|SITE= <scene name='pdbsite=AC1:Glc+Binding+Site+For+Residue+A+721'>AC1</scene>, <scene name='pdbsite=AC2:Glc+Binding+Site+For+Residue+A+722'>AC2</scene>, <scene name='pdbsite=AC3:Glc+Binding+Site+For+Residue+A+723'>AC3</scene>, <scene name='pdbsite=AC4:Glc+Binding+Site+For+Residue+B+731'>AC4</scene>, <scene name='pdbsite=AC5:Glc+Binding+Site+For+Residue+B+732'>AC5</scene>, <scene name='pdbsite=AC6:Glc+Binding+Site+For+Residue+B+733'>AC6</scene>, <scene name='pdbsite=AC7:Glc+Binding+Site+For+Residue+A+735'>AC7</scene>, <scene name='pdbsite=AC8:Glc+Binding+Site+For+Residue+A+736'>AC8</scene>, <scene name='pdbsite=AC9:Ca+Binding+Site+For+Residue+A+741'>AC9</scene>, <scene name='pdbsite=BC1:Ca+Binding+Site+For+Residue+B+742'>BC1</scene>, <scene name='pdbsite=BC2:Act+Binding+Site+For+Residue+A+751'>BC2</scene>, <scene name='pdbsite=BC3:Act+Binding+Site+For+Residue+B+752'>BC3</scene>, <scene name='pdbsite=BC4:Gol+Binding+Site+For+Residue+A+761'>BC4</scene> and <scene name='pdbsite=BC5:Gol+Binding+Site+For+Residue+B+762'>BC5</scene>
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Pullulanase Pullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.41 3.2.1.41]
|GENE= amyX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
}}
 
'''Crystal structure of pullulanase type I from Bacillus subtilis str. 168 complexed with maltose'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
2E9B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pullulanase Pullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.41 3.2.1.41] Known structural/functional Sites: <scene name='pdbsite=AC1:Glc+Binding+Site+For+Residue+A+721'>AC1</scene>, <scene name='pdbsite=AC2:Glc+Binding+Site+For+Residue+A+722'>AC2</scene>, <scene name='pdbsite=AC3:Glc+Binding+Site+For+Residue+A+723'>AC3</scene>, <scene name='pdbsite=AC4:Glc+Binding+Site+For+Residue+B+731'>AC4</scene>, <scene name='pdbsite=AC5:Glc+Binding+Site+For+Residue+B+732'>AC5</scene>, <scene name='pdbsite=AC6:Glc+Binding+Site+For+Residue+B+733'>AC6</scene>, <scene name='pdbsite=AC7:Glc+Binding+Site+For+Residue+A+735'>AC7</scene>, <scene name='pdbsite=AC8:Glc+Binding+Site+For+Residue+A+736'>AC8</scene>, <scene name='pdbsite=AC9:Ca+Binding+Site+For+Residue+A+741'>AC9</scene>, <scene name='pdbsite=BC1:Ca+Binding+Site+For+Residue+B+742'>BC1</scene>, <scene name='pdbsite=BC2:Act+Binding+Site+For+Residue+A+751'>BC2</scene>, <scene name='pdbsite=BC3:Act+Binding+Site+For+Residue+B+752'>BC3</scene>, <scene name='pdbsite=BC4:Gol+Binding+Site+For+Residue+A+761'>BC4</scene> and <scene name='pdbsite=BC5:Gol+Binding+Site+For+Residue+B+762'>BC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E9B OCA].  
2E9B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E9B OCA].  


==Reference==
==Reference==
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168., Malle D, Itoh T, Hashimoto W, Murata K, Utsumi S, Mikami B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):381-4. Epub 2006 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16582490 16582490]
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168., Malle D, Itoh T, Hashimoto W, Murata K, Utsumi S, Mikami B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):381-4. Epub 2006 Mar 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16582490 16582490]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Pullulanase]]
[[Category: Pullulanase]]
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[[Category: smultiple domain]]
[[Category: smultiple domain]]


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