4dam: Difference between revisions
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==Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor== | |||
<StructureSection load='4dam' size='340' side='right' caption='[[4dam]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4dam]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DAM FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3eiv|3eiv]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCC61A.04c, SCO2683, ssb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dam OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dam RCSB], [http://www.ebi.ac.uk/pdbsum/4dam PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Following mutational analysis, we concluded that ssbA is essential, whereas ssbB plays a key role in chromosome segregation during sporulation. In the ssbB mutant, approximately 30% of spores lacked DNA. The two ssb genes were expressed differently; in minimal medium, gene expression was prolonged for both genes and significantly upregulated for ssbB. The ssbA gene is transcribed as part of a polycistronic mRNA from two initiation sites, 163 bp and 75 bp upstream of the rpsF translational start codon. The ssbB gene is transcribed as a monocistronic mRNA, from an unusual promoter region, 73 bp upstream of the AUG codon. Distinctive DNA-binding affinities of single-stranded DNA-binding proteins monitored by tryptophan fluorescent quenching and electrophoretic mobility shift were observed. The crystal structure of SsbB at 1.7 A resolution revealed a common OB-fold, lack of the clamp-like structure conserved in SsbA and previously unpublished S-S bridges between the A/B and C/D subunits. This is the first report of the determination of paralogous single-stranded DNA-binding protein structures from the same organism. Phylogenetic analysis revealed frequent duplication of ssb genes in Actinobacteria, whereas their strong retention suggests that they are involved in important cellular functions. | |||
Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.,Paradzik T, Ivic N, Filic Z, Manjasetty BA, Herron P, Luic M, Vujaklija D Nucleic Acids Res. 2013 Apr 1;41(6):3659-72. doi: 10.1093/nar/gkt050. Epub 2013, Feb 7. PMID:23393191<ref>PMID:23393191</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
[[ | </div> | ||
==See Also== | |||
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Streptomyces coelicolor]] | [[Category: Streptomyces coelicolor]] | ||
[[Category: Filic, Z | [[Category: Filic, Z]] | ||
[[Category: Herron, P | [[Category: Herron, P]] | ||
[[Category: Ivic, N | [[Category: Ivic, N]] | ||
[[Category: Luic, M | [[Category: Luic, M]] | ||
[[Category: Manjasetty, B A | [[Category: Manjasetty, B A]] | ||
[[Category: Paradzik, T | [[Category: Paradzik, T]] | ||
[[Category: Vujaklija, D | [[Category: Vujaklija, D]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Dna-binding]] | [[Category: Dna-binding]] | ||
[[Category: Ob-fold]] | [[Category: Ob-fold]] | ||
[[Category: Single-stranded dna]] | [[Category: Single-stranded dna]] | ||
Revision as of 08:57, 21 December 2014
Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor
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