3m66: Difference between revisions

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{{STRUCTURE_3m66| PDB=3m66 | SCENE= }}
==Crystal structure of human Mitochondrial Transcription Termination Factor 3==
===Crystal structure of human Mitochondrial Transcription Termination Factor 3===
<StructureSection load='3m66' size='340' side='right' caption='[[3m66]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
{{ABSTRACT_PUBMED_20430012}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3m66]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M66 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M66 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CGI-12, MTERFD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m66 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m66 RCSB], [http://www.ebi.ac.uk/pdbsum/3m66 PDBsum]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m6/3m66_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In mammalian cells, a family of mitochondrial transcription termination factors (MTERFs) regulates mitochondrial gene expression. MTERF family members share a approximately 270 residues long MTERF-domain required for DNA binding and transcription regulation. However, the structure of this widely conserved domain is unknown. Here, we show that the MTERF-domain of human MTERF3 forms a half-doughnut-shaped right-handed superhelix. The superhelix is built from alpha-helical tandem repeats that display a novel triangular three-helix motif. This repeat motif, which we denote the MTERF-motif, is a conserved structural element present in proteins from metazoans, plants, and protozoans. Furthermore, a narrow, strongly positively charged nucleic acid-binding path is found in the middle of the concave side of the half-doughnut. This arrangement suggests a half clamp nucleic acid-binding mode for MTERF-domains.


==Function==
Structure of mitochondrial transcription termination factor 3 reveals a novel nucleic acid-binding domain.,Spahr H, Samuelsson T, Hallberg BM, Gustafsson CM Biochem Biophys Res Commun. 2010 Jul 2;397(3):386-90. Epub 2010 Apr 27. PMID:20430012<ref>PMID:20430012</ref>
[[http://www.uniprot.org/uniprot/MTER1_HUMAN MTER1_HUMAN]] Binds promoter DNA and regulates initiation of transcription. Required for normal mitochondrial transcription, and for normal assembly of mitochondrial respiratory complexes. Required for normal mitochondrial function (By similarity).<ref>PMID:17662942</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[3m66]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M66 OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020430012</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Gustafsson, C M.]]
[[Category: Gustafsson, C M]]
[[Category: Hallberg, B M.]]
[[Category: Hallberg, B M]]
[[Category: Samuelsson, T.]]
[[Category: Samuelsson, T]]
[[Category: Spahr, H.]]
[[Category: Spahr, H]]
[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
[[Category: Mitochondrion]]
[[Category: Mitochondrion]]

Revision as of 17:22, 18 December 2014

Crystal structure of human Mitochondrial Transcription Termination Factor 3

3m66, resolution 1.60Å

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