3n6o: Difference between revisions

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{{STRUCTURE_3n6o|  PDB=3n6o  |  SCENE=  }}
==Crystal structure of the GEF and P4M domain of DrrA/SidM from Legionella pneumophila==
===Crystal structure of the GEF and P4M domain of DrrA/SidM from Legionella pneumophila===
<StructureSection load='3n6o' size='340' side='right' caption='[[3n6o]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
{{ABSTRACT_PUBMED_20616805}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3n6o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N6O FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DrrA, lpg2464 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 Legionella pneumophila])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n6o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n6o RCSB], [http://www.ebi.ac.uk/pdbsum/3n6o PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The DrrA protein of Legionella pneumophila is involved in mistargeting of endoplasmic reticulum-derived vesicles to Legionella-containing vacuoles through recruitment of the small GTPase Rab1. To this effect, DrrA binds specifically to phosphatidylinositol 4-phosphate (PtdIns(4)P) lipids on the cytosolic surface of the phagosomal membrane shortly after infection. In this study, we present the atomic structure of the PtdIns(4)P-binding domain of a protein (DrrA) from a human pathogen. A detailed kinetic investigation of its interaction with PtdIns(4)P reveals that DrrA binds to this phospholipid with, as yet unprecedented, high affinity, suggesting that DrrA can sense a very low abundance of the lipid.


==Function==
High-affinity binding of phosphatidylinositol 4-phosphate by Legionella pneumophila DrrA.,Schoebel S, Blankenfeldt W, Goody RS, Itzen A EMBO Rep. 2010 Aug;11(8):598-604. Epub 2010 Jul 9. PMID:20616805<ref>PMID:20616805</ref>
[[http://www.uniprot.org/uniprot/Q5ZSQ3_LEGPH Q5ZSQ3_LEGPH]] Virulence effector that plays a key role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a GDP-GTP exchange factor (GEF) for the small GTPase Rab1 (RAB1A, RAB1B or RAB1C), thereby converting Rab1 to an active GTP-bound state, leading to the incorporation of Rab1 into LCVs. Also shows RabGDI displacement factor (GDF) activity; however, this probably represents a passive activity following the GEF activity. Also acts as an adenylyltransferase by mediating the addition of adenosine 5'-monophosphate (AMP) to 'Tyr-77' of host RAB1B, thereby rendering RAB1B constitutively active. Also has adenylyltransferase activity towards Rab6 and Rab35. Also displays guanylyltransferase activity by mediating the addition of guanosine 5'-monophosphate (GMP) to host RAB1B in vitro; however such activity remains uncertain in vivo. Specifically binds phosphatidylinositol 4-phosphate (PtdIns(4)P) lipids on the cytosolic surface of the phagosomal membrane shortly after infection.<ref>PMID:16824952</ref> <ref>PMID:17947549</ref> <ref>PMID:21822290</ref> <ref>PMID:20064470</ref> <ref>PMID:19942850</ref> <ref>PMID:20176951</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[3n6o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N6O OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020616805</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Legionella pneumophila]]
[[Category: Legionella pneumophila]]
[[Category: Blankenfeldt, W.]]
[[Category: Blankenfeldt, W]]
[[Category: Goody, R S.]]
[[Category: Goody, R S]]
[[Category: Itzen, A.]]
[[Category: Itzen, A]]
[[Category: Schoebel, S.]]
[[Category: Schoebel, S]]
[[Category: Gef]]
[[Category: Gef]]
[[Category: Lcv]]
[[Category: Lcv]]
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