1qni: Difference between revisions
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==Overview== | ==Overview== | ||
Nitrous oxide (N20) is a greenhouse gas, the third most significant, contributor to global warming. As a key process for N20 elimination from, the biosphere, N20 reductases catalyze the two-electron reduction of N20, to N2. These 2 x 65 kDa copper enzymes are thought to contain a CuA, electron entry site, similar to that of cytochrome c oxidase, and a CuZ, catalytic center. The copper anomalous signal was used to solve the, crystal structure of N20 reductase from Pseudomonas nautica by, multiwavelength anomalous dispersion, to a resolution of 2.4 A. The, structure reveals that the CuZ center belongs to a new type of metal, cluster, in which four copper ions are liganded by seven histidine, residues. N20 binds to this center via a single copper ion. The remaining, copper ions might act as . | Nitrous oxide (N20) is a greenhouse gas, the third most significant, contributor to global warming. As a key process for N20 elimination from, the biosphere, N20 reductases catalyze the two-electron reduction of N20, to N2. These 2 x 65 kDa copper enzymes are thought to contain a CuA, electron entry site, similar to that of cytochrome c oxidase, and a CuZ, catalytic center. The copper anomalous signal was used to solve the, crystal structure of N20 reductase from Pseudomonas nautica by, multiwavelength anomalous dispersion, to a resolution of 2.4 A. The, structure reveals that the CuZ center belongs to a new type of metal, cluster, in which four copper ions are liganded by seven histidine, residues. N20 binds to this center via a single copper ion. The remaining, copper ions might act as an electron reservoir, assuring a fast electron, transfer and avoiding the formation of dead-end products. | ||
==About this Structure== | ==About this Structure== | ||
1QNI is a | 1QNI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Marinobacter_hydrocarbonoclasticus Marinobacter hydrocarbonoclasticus] with CA, CL, CUA and CUZ as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Sites: CUA, CUB, CUC, CUD, CUE, CUF, CUU, CUV, CUW, CUX, CUY and CUZ. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QNI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: mad]] | [[Category: mad]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:34:46 2007'' | ||
