3p0b: Difference between revisions

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{{STRUCTURE_3p0b|  PDB=3p0b  |  SCENE=  }}
==Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed==
===Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed===
<StructureSection load='3p0b' size='340' side='right' caption='[[3p0b]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
{{ABSTRACT_PUBMED_021097495}}
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3p0b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P0B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P0B FirstGlance]. <br>
==Function==
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
[[http://www.uniprot.org/uniprot/P84162_THETH P84162_THETH]] Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin).
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ufa|1ufa]]</td></tr>
 
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tthHB8IM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p0b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p0b RCSB], [http://www.ebi.ac.uk/pdbsum/3p0b PDBsum]</span></td></tr>
[[3p0b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P0B OCA].  
</table>
__TOC__
</StructureSection>
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Dijkstra, B W.]]
[[Category: Dijkstra, B W]]
[[Category: Pijning, T.]]
[[Category: Pijning, T]]
[[Category: Glycogen branching]]
[[Category: Glycogen branching]]
[[Category: Glycoside hydrolase gh57]]
[[Category: Glycoside hydrolase gh57]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 06:48, 19 December 2014

Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed

3p0b, resolution 1.35Å

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