G1SecL05: Difference between revisions

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scene='Insert optional scene name here' />

===Trp-216===

===Trp-216===One hyper-variable region is the Tryptophan amino acid residue sequence 216. Polar amino acid side chains flank the Tryptophan, and changes in such amino acids can ultimately affect the reactivity of OspA to monoclonal antibodies. Differences in side chains of amino acids on the polar surface of a helical structure surrounding the highly conserved tryptophan residue 216 can affect the reactivity of OspA.

===Ala-208===

===Ala-208===In B. burgdorferi, residue 208 expresses Alanine, whereas in B. afzelii, and B. garinii, Glutamine is expressed. The variation in this residue is another factor, which inhibits successful antibody cross-reactivity between other strains of Borrelia. LA-2 and OspA of B. burgdorferi forms a tight border when binding and this is promoted by the Alanine 208 residue. The Glutamine residue that is found in B. afzelii and B. garinii is longer and more problematic when it comes to binding. It creates a looser, more ineffective interface between the LA-2 and OspA, which conclusively makes an ineffectual vaccine.

===Residues 217-237===

===Residues 217-237===The binding of OspA and the LA-2 FAB is defined by the amino acid residue sequence 217-237, and causes nonspecific binding thus inhibiting the LA2-FAB-OspA complex binding to other species of Borrelia.

<scene name='G1SecL05/1fj1-condensed/4'>Three variable regions highlighted Tryptophan 216 - Blue, Alanine 208 - Purple, Highly Variable LA-2 Fab + OspA conjunction - Red</scene>

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@@ Line 31: / Line 31: @@
   scene='Insert optional scene name here' />
-===Trp-216===
+===Trp-216===One hyper-variable region is the Tryptophan amino acid residue sequence 216. Polar amino acid side chains flank the Tryptophan, and changes in such amino acids can ultimately affect the reactivity of OspA to monoclonal antibodies. Differences in side chains of amino acids on the polar surface of a helical structure surrounding the highly conserved tryptophan residue 216 can affect the reactivity of OspA.
-===Ala-208===
+===Ala-208===In B. burgdorferi, residue 208 expresses Alanine, whereas in B. afzelii, and B. garinii, Glutamine is expressed. The variation in this residue is another factor, which inhibits successful antibody cross-reactivity between other strains of Borrelia. LA-2 and OspA of B. burgdorferi forms a tight border when binding and this is promoted by the Alanine 208 residue. The Glutamine residue that is found in B. afzelii and B. garinii is longer and more problematic when it comes to binding. It creates a looser, more ineffective interface between the LA-2 and OspA, which conclusively makes an ineffectual vaccine.
-===Residues 217-237===
+===Residues 217-237===The binding of OspA and the LA-2 FAB is defined by the amino acid residue sequence 217-237, and causes nonspecific binding thus inhibiting the LA2-FAB-OspA complex binding to other species of Borrelia.
 <scene name='G1SecL05/1fj1-condensed/4'>Three variable regions highlighted Tryptophan 216 - Blue, Alanine 208 - Purple, Highly Variable LA-2 Fab + OspA conjunction - Red</scene>