2fvy: Difference between revisions
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'''High Resolution Glucose Bound Crystal Structure of GGBP''' | {{Structure | ||
|PDB= 2fvy |SIZE=350|CAPTION= <scene name='initialview01'>2fvy</scene>, resolution 0.92Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= mglB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''High Resolution Glucose Bound Crystal Structure of GGBP''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2FVY is a [ | 2FVY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FVY OCA]. | ||
==Reference== | ==Reference== | ||
Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures., Borrok MJ, Kiessling LL, Forest KT, Protein Sci. 2007 Jun;16(6):1032-41. Epub 2007 May 1. PMID:[http:// | Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures., Borrok MJ, Kiessling LL, Forest KT, Protein Sci. 2007 Jun;16(6):1032-41. Epub 2007 May 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17473016 17473016] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:57:22 2008'' | ||
Revision as of 14:57, 20 March 2008
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| 2fvy, resolution 0.92Å | |||||||||||||
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| Ligands: | GLC, CA, ACT, CO2 and GOL | ||||||||||||
| Gene: | mglB (Escherichia coli) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
High Resolution Glucose Bound Crystal Structure of GGBP
Overview
D-Glucose/D-Galactose-binding protein (GGBP) mediates chemotaxis toward and active transport of glucose and galactose in a number of bacterial species. GGBP, like other periplasmic binding proteins, can exist in open (ligand-free) and closed (ligand-bound) states. We report a 0.92 angstroms resolution structure of GGBP from Escherichia coli in the glucose-bound state and the first structure of an open, unbound form of GGBP (at 1.55 angstroms resolution). These structures vary in the angle between the two structural domains; the observed difference of 31 degrees arises from torsion angle changes in a three-segment hinge. A comparison with the closely related periplasmic receptors, ribose- and allose-binding proteins, shows that the GGBP hinge residue positions that undergo the largest conformational changes are different. Furthermore, the high-quality data collected for the atomic resolution glucose-bound structure allow for the refinement of specific hydrogen atom positions, the assignment of alternate side chain conformations, the first description of CO(2) trapped after radiation-induced decarboxylation, and insight into the role of the exo-anomeric effect in sugar binding. Together, these structures provide insight into how the hinge-bending movement of GGBP facilitates ligand binding, transport, and signaling.
About this Structure
2FVY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures., Borrok MJ, Kiessling LL, Forest KT, Protein Sci. 2007 Jun;16(6):1032-41. Epub 2007 May 1. PMID:17473016
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