2g1g: Difference between revisions
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[[Image:2g1g.gif|left|200px]] | [[Image:2g1g.gif|left|200px]] | ||
'''Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine''' | {{Structure | ||
|PDB= 2g1g |SIZE=350|CAPTION= <scene name='initialview01'>2g1g</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2G1G is a [ | 2G1G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1G OCA]. | ||
==Reference== | ==Reference== | ||
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:[http:// | The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16738127 16738127] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Lescrinier, E.]] | [[Category: Lescrinier, E.]] | ||
[[Category: t-rnai anticodonloop t6a]] | [[Category: t-rnai anticodonloop t6a]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:59:16 2008'' | ||
Revision as of 14:59, 20 March 2008
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Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine
Overview
Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.
About this Structure
2G1G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:16738127
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