3pkm: Difference between revisions
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==Crystal structure of Cas6 with its substrate RNA== | |||
=== | <StructureSection load='3pkm' size='340' side='right' caption='[[3pkm]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3pkm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PKM FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pkm RCSB], [http://www.ebi.ac.uk/pdbsum/3pkm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) found in prokaryotic genomes confer small RNA-mediated protection against viruses and other invaders. CRISPR loci contain iterations of a short repeat sequence alternating with small segments of varying invader-derived sequences. Distinct families of CRISPR-associated Cas proteins function to cleave within the repeat sequence of CRISPR transcripts and produce the individual invader-targeting crRNAs. Here, we report the crystal structure of Pyrococcus furiosus Cas6 bound with a repeat RNA at 3.2 A resolution. In contrast to other Cas families of endonucleases, Cas6 clasps nucleotides 2-9 of the repeat RNA using its two ferredoxin-like domains, and the enzyme-anchored 5' end tethers the distal cleavage site of the RNA between nucleotides 22 and 23 to the predicted enzyme active site on the opposite side of the ferrodoxin-like domains. Our findings suggest a wrap-around mechanism for CRISPR RNA recognition and cleavage by Cas6 and related processing endonucleases. | |||
Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and Cleavage.,Wang R, Preamplume G, Terns MP, Terns RM, Li H Structure. 2011 Feb 9;19(2):257-64. PMID:21300293<ref>PMID:21300293</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Li, H | [[Category: Li, H]] | ||
[[Category: Preamplume, G | [[Category: Preamplume, G]] | ||
[[Category: Wang, R | [[Category: Wang, R]] | ||
[[Category: Cas6]] | [[Category: Cas6]] | ||
[[Category: Crispr]] | [[Category: Crispr]] | ||
Revision as of 10:40, 19 December 2014
Crystal structure of Cas6 with its substrate RNA
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