3qd7: Difference between revisions
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==Crystal structure of YdaL, a stand-alone small MutS-related protein from Escherichia coli== | |||
<StructureSection load='3qd7' size='340' side='right' caption='[[3qd7]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3qd7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QD7 FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ydaL, b1340, JW1334 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qd7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qd7 RCSB], [http://www.ebi.ac.uk/pdbsum/3qd7 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sequence homologs of the small MutS-related (Smr) domain, the C-terminal endonuclease domain of MutS2, also exist as stand-alone proteins. In this study, we report the crystal structure of a proteolyzed fragment of YdaL (YdaL-), a stand-alone Smr protein from Escherichia coli. In this structure, residues 86-170 assemble into a classical Smr core domain and are embraced by an N-terminal extension (residues 40-85) with an alpha/beta/alpha fold. Sequence alignment indicates that the N-terminal extension is conserved among a number of stand-alone Smr proteins, suggesting structural diversity among Smr domains. We also discovered that the DNA binding affinity and endonuclease activity of the truncated YdaL- protein were slightly lower than those of full-length YdaL-, suggesting that residues 1-38 may be involved in DNA binding. | |||
Crystal structure of YdaL, a stand-alone small MutS-related protein from Escherichia coli.,Gui WJ, Qu QH, Chen YY, Wang M, Zhang XE, Bi LJ, Jiang T J Struct Biol. 2011 May;174(2):282-9. Epub 2011 Jan 27. PMID:21276852<ref>PMID:21276852</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Bi, L J | [[Category: Bi, L J]] | ||
[[Category: Chen, Y Y | [[Category: Chen, Y Y]] | ||
[[Category: Gui, W J | [[Category: Gui, W J]] | ||
[[Category: Jiang, T | [[Category: Jiang, T]] | ||
[[Category: Qu, Q H | [[Category: Qu, Q H]] | ||
[[Category: Wang, M | [[Category: Wang, M]] | ||
[[Category: Zhang, X E | [[Category: Zhang, X E]] | ||
[[Category: Alpha/beta/alpha fold]] | [[Category: Alpha/beta/alpha fold]] | ||
[[Category: Endonuclease]] | [[Category: Endonuclease]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
