1uo6: Difference between revisions
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==Overview== | ==Overview== | ||
Complete and highly redundant data sets were collected at nine different, wavelengths between 0.80 and 2.65 A on a xenon derivative of porcine, pancreatic elastase in both air and helium atmospheres. The magnitude of, the anomalous signal, as assessed by the xenon-peak height in the, anomalous difference Patterson synthesis, is affected by the wavelength of, data collection as well as by the scaling model used. For data collected, at wavelengths longer than 1.7 A, the use of a three-dimensional scaling, protocol is essential in order to obtain the highest possible anomalous, signal. Based on the scaling protocols currently available, the optimal, wavelength range for data collection appears to be between 2.1 and 2.4 A., Beyond that, any further increase in signal will be compensated for . | Complete and highly redundant data sets were collected at nine different, wavelengths between 0.80 and 2.65 A on a xenon derivative of porcine, pancreatic elastase in both air and helium atmospheres. The magnitude of, the anomalous signal, as assessed by the xenon-peak height in the, anomalous difference Patterson synthesis, is affected by the wavelength of, data collection as well as by the scaling model used. For data collected, at wavelengths longer than 1.7 A, the use of a three-dimensional scaling, protocol is essential in order to obtain the highest possible anomalous, signal. Based on the scaling protocols currently available, the optimal, wavelength range for data collection appears to be between 2.1 and 2.4 A., Beyond that, any further increase in signal will be compensated for or, even superseded by a concomitant increase in noise, which cannot be fully, corrected for. Data collection in a helium atmosphere yields higher, I/sigma(I) values, but not significantly better anomalous differences, than data collection in air. | ||
==About this Structure== | ==About this Structure== | ||
1UO6 is a | 1UO6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4, CL, NA, XE and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UO6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: xenon binding]] | [[Category: xenon binding]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:33:37 2007'' | ||
