4han: Difference between revisions
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==Crystal structure of Galectin 8 with NDP52 peptide== | |||
=== | <StructureSection load='4han' size='340' side='right' caption='[[4han]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4han]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alfaropsis_roxburghiana Alfaropsis roxburghiana] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HAN FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CALCOCO2, NDP52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=139932 Alfaropsis roxburghiana])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4han FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4han OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4han RCSB], [http://www.ebi.ac.uk/pdbsum/4han PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Infectious bacteria are cleared from mammalian cells by host autophagy in combination with other upstream cellular components, such as the autophagic receptor NDP52 and sugar receptor galectin-8. However, the detailed molecular basis of the interaction between these two receptors remains to be elucidated. Here, we report the biochemical characterization of both NDP52 and galectin-8 as well as the crystal structure of galectin-8 complexed with an NDP52 peptide. The unexpected observation of nicotinamide adenine dinucleotide located at the carbohydrate-binding site expands our knowledge of the sugar-binding specificity of galectin-8. The NDP52-galectin-8 complex structure explains the key determinants for recognition on both receptors and defines a special orientation of N- and C-terminal carbohydrate recognition domains of galectin-8. Dimeric NDP52 forms a ternary complex with two monomeric galectin-8 molecules as well as two LC3C molecules. These results lay the groundwork for understanding how host cells target bacterial pathogens for autophagy. | |||
Structural basis for recognition of autophagic receptor NDP52 by the sugar receptor galectin-8.,Kim BW, Hong SB, Kim JH, Kwon do H, Song HK Nat Commun. 2013;4:1613. doi: 10.1038/ncomms2606. PMID:23511477<ref>PMID:23511477</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | ==See Also== | ||
<references | *[[Galectin|Galectin]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Alfaropsis roxburghiana]] | [[Category: Alfaropsis roxburghiana]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Hong, S B | [[Category: Hong, S B]] | ||
[[Category: Kim, B W | [[Category: Kim, B W]] | ||
[[Category: Kim, J H | [[Category: Kim, J H]] | ||
[[Category: Kwon, D H | [[Category: Kwon, D H]] | ||
[[Category: Song, H K | [[Category: Song, H K]] | ||
[[Category: Autophagy]] | [[Category: Autophagy]] | ||
[[Category: Autophagy adapter molecule]] | [[Category: Autophagy adapter molecule]] | ||
Revision as of 12:00, 21 December 2014
Crystal structure of Galectin 8 with NDP52 peptide
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