1uoz: Difference between revisions
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==Overview== | ==Overview== | ||
The genomes of various Mycobacterium tuberculosis strains encode proteins, that do not appear to play a role in the growth or survival of the, bacterium in its mammalian host, including some implicated in plant cell, wall breakdown. Here we show that M. tuberculosis H37Rv does indeed, possess a functional cellulase. The x-ray crystal structure of this, enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a, highly conserved substrate-binding cleft, which affords similar, and, unusual, distortion of the substrate at the catalytic center. The, endoglucanase activity, together with the existence of a putative, membrane-associated crystalline polysaccharide-binding protein, may, reflect the ancestral soil origin of the Mycobacterium or hint at a, previously unconsidered . | The genomes of various Mycobacterium tuberculosis strains encode proteins, that do not appear to play a role in the growth or survival of the, bacterium in its mammalian host, including some implicated in plant cell, wall breakdown. Here we show that M. tuberculosis H37Rv does indeed, possess a functional cellulase. The x-ray crystal structure of this, enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a, highly conserved substrate-binding cleft, which affords similar, and, unusual, distortion of the substrate at the catalytic center. The, endoglucanase activity, together with the existence of a putative, membrane-associated crystalline polysaccharide-binding protein, may, reflect the ancestral soil origin of the Mycobacterium or hint at a, previously unconsidered environmental niche. | ||
==About this Structure== | ==About this Structure== | ||
1UOZ is a | 1UOZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with GLC, SSG, SGC, SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: glycoside hydrolase]] | [[Category: glycoside hydrolase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:33:48 2007'' | ||
