2h95: Difference between revisions
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'''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy''' | {{Structure | ||
|PDB= 2h95 |SIZE=350|CAPTION= <scene name='initialview01'>2h95</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
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}} | |||
'''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2H95 is a [ | 2H95 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H95 OCA]. | ||
==Reference== | ==Reference== | ||
Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:[http:// | Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17384070 17384070] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Asbury, T.]] | [[Category: Asbury, T.]] | ||
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[[Category: protein-ligand]] | [[Category: protein-ligand]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:14:11 2008'' | ||
Revision as of 15:14, 20 March 2008
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Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy
Overview
Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine.
About this Structure
2H95 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:17384070
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