4hqm: Difference between revisions
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==The crystal structure of QsrR-menadione complex== | |||
=== | <StructureSection load='4hqm' size='340' side='right' caption='[[4hqm]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hqm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HQM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HQM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=17Z:2-METHYLNAPHTHALENE-1,4-DIOL'>17Z</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hqm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hqm RCSB], [http://www.ebi.ac.uk/pdbsum/4hqm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Quinone molecules are intracellular electron-transport carriers, as well as critical intra- and extracellular signals. However, transcriptional regulation of quinone signaling and its molecular basis are poorly understood. Here, we identify a thiol-stress-sensing regulator YodB family transcriptional regulator as a central component of quinone stress response of Staphylococcus aureus, which we have termed the quinone-sensing and response repressor (QsrR). We also identify and confirm an unprecedented quinone-sensing mechanism based on the S-quinonization of the essential residue Cys-5. Structural characterizations of the QsrR-DNA and QsrR-menadione complexes further reveal that the covalent association of menadione directly leads to the release of QsrR from operator DNA following a 10 degrees rigid-body rotation as well as a 9-A elongation between the dimeric subunits. The molecular level characterization of this quinone-sensing transcriptional regulator provides critical insights into quinone-mediated gene regulation in human pathogens. | |||
Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR.,Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, Cho H, Brugarolas P, Gao YN, Peterson SN, Lan L, Bae T, He C Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5010-5. doi:, 10.1073/pnas.1219446110. Epub 2013 Mar 11. PMID:23479646<ref>PMID:23479646</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Bae, T | [[Category: Bae, T]] | ||
[[Category: Brugarolas, P | [[Category: Brugarolas, P]] | ||
[[Category: Deng, X | [[Category: Deng, X]] | ||
[[Category: Gao, N | [[Category: Gao, N]] | ||
[[Category: He, C | [[Category: He, C]] | ||
[[Category: Ji, Q | [[Category: Ji, Q]] | ||
[[Category: Jones, M B | [[Category: Jones, M B]] | ||
[[Category: Lan, L | [[Category: Lan, L]] | ||
[[Category: Liang, H | [[Category: Liang, H]] | ||
[[Category: Peterson, S N | [[Category: Peterson, S N]] | ||
[[Category: Sun, F | [[Category: Sun, F]] | ||
[[Category: Zhang, L | [[Category: Zhang, L]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Menadione-modified protein]] | [[Category: Menadione-modified protein]] | ||
[[Category: Transcription regulator]] | [[Category: Transcription regulator]] | ||
