4jz8: Difference between revisions

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{{STRUCTURE_4jz8| PDB=4jz8 | SCENE= }}
==Carbamate kinase from Giardia lamblia bound to citric acid==
===Carbamate kinase from Giardia lamblia bound to citric acid===
<StructureSection load='4jz8' size='340' side='right' caption='[[4jz8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
{{ABSTRACT_PUBMED_23700444}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4jz8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_lamblia_atcc_50803 Giardia lamblia atcc 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JZ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JZ8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kzf|3kzf]], [[4jz7|4jz7]], [[4jz9|4jz9]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GL50803_16453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=184922 Giardia lamblia ATCC 50803])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbamate_kinase Carbamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.2 2.7.2.2] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jz8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jz8 RCSB], [http://www.ebi.ac.uk/pdbsum/4jz8 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The parasite Giardia lamblia utilizes the L-arginine dihydrolase pathway to generate ATP from L-arginine. Carbamate kinase (CK) catalyzes the last step in this pathway, converting ADP and carbamoyl phosphate to ATP and ammonium carbamate. Because the L-arginine pathway is essential for G. lamblia survival and absent in high eukaryotes including humans, the enzyme is a potential target for drug development. We have determined two crystal structures of G. lamblia CK (glCK) with bound ligands. One structure, in complex with a nonhydrolyzable ATP analog, adenosine 5'-adenylyl-beta,gamma-imidodiphosphate (AMP-PNP), was determined at 2.6 A resolution. The second structure, in complex with citric acid bound in the postulated carbamoyl phosphate binding site, was determined in two slightly different states at 2.1 and 2.4 A resolution. These structures reveal conformational flexibility of an auxiliary domain (amino acid residues 123-170), which exhibits open or closed conformations or structural disorder, depending on the bound ligand. The structures also reveal a smaller conformational change in a region associated the AMP-PNP adenine binding site. The protein residues involved in binding, together with a model of the transition state, suggest that catalysis follows an in-line, predominantly dissociative, phosphotransfer reaction mechanism, and that closure of the flexible auxiliary domain is required to protect the transition state from bulk solvent.


==About this Structure==
Crystal Structures of Carbamate Kinase from Giardia lamblia Bound with Citric Acid and AMP-PNP.,Lim K, Kulakova L, Galkin A, Herzberg O PLoS One. 2013 May 20;8(5):e64004. doi: 10.1371/journal.pone.0064004. Print 2013. PMID:23700444<ref>PMID:23700444</ref>
[[4jz8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_lamblia_atcc_50803 Giardia lamblia atcc 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JZ8 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023700444</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Carbamate kinase]]
[[Category: Carbamate kinase]]
[[Category: Giardia lamblia atcc 50803]]
[[Category: Giardia lamblia atcc 50803]]
[[Category: Herzberg, O.]]
[[Category: Herzberg, O]]
[[Category: Lim, K.]]
[[Category: Lim, K]]
[[Category: Adp]]
[[Category: Adp]]
[[Category: Atp carbamate phosphotransferase]]
[[Category: Atp carbamate phosphotransferase]]
Retrieved from "https://proteopedia.org/4jz8"