2hnk: Difference between revisions
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'''Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans''' | {{Structure | ||
|PDB= 2hnk |SIZE=350|CAPTION= <scene name='initialview01'>2hnk</scene>, resolution 2.300Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> and <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2HNK is a [ | 2HNK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HNK OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:[http:// | Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17561415 17561415] | ||
[[Category: Leptospira interrogans]] | [[Category: Leptospira interrogans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:19:16 2008'' | ||
Revision as of 15:19, 20 March 2008
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| 2hnk, resolution 2.300Å | |||||||||||||
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| Ligands: | SO4, SAH and PEG | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans
Overview
The S-adenosylmethionine (SAM)-dependent O-methyltransferase from Leptospira interrogans (LiOMT) expressed by gene LA0415 belongs to the Methyltransf_3 family (Pfam PF01596). In this family all of the five bacterial homologues with known function are reported as SAM-dependent O-methylstransferases involved in antibiotic production. The crystal structure of LiOMT in complex with S-adenosylhomocysteine reported here is the first bacterial protein structure in this family. The LiOMT structure shows a conserved SAM-binding region and a probable metal-dependent catalytic site. The molecules of LiOMT generate homodimers by N-terminal swapping, which assists the pre-organization of the substrate-binding site. Based on the sequence and structural analysis, it is implied by the catalytic and substrate-binding site that the substrate of LiOMT is a phenolic derivative, which probably has a large ring-shaped moiety.
About this Structure
2HNK is a Single protein structure of sequence from Leptospira interrogans. Full crystallographic information is available from OCA.
Reference
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:17561415
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