1uvn: Difference between revisions
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==Overview== | ==Overview== | ||
The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA, from the three segments of the dsRNA viral genome. We have cocrystallized, RNA oligonucleotides with the polymerase, revealing the mode of binding of, RNA templates. This binding is somewhat different from that previously, seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase, complex by the addition of substrate and Mg2+ initiates a single round of, reaction within the crystal to form a dead-end complex that partially, collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we, have been able to capture the inhibited complex which shows distortion, that explains the structural basis for the inhibition of such . | The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA, from the three segments of the dsRNA viral genome. We have cocrystallized, RNA oligonucleotides with the polymerase, revealing the mode of binding of, RNA templates. This binding is somewhat different from that previously, seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase, complex by the addition of substrate and Mg2+ initiates a single round of, reaction within the crystal to form a dead-end complex that partially, collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we, have been able to capture the inhibited complex which shows distortion, that explains the structural basis for the inhibition of such polymerases, by Ca2+. | ||
==About this Structure== | ==About this Structure== | ||
1UVN is a | 1UVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CA, MN and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UVN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:30:33 2007'' | ||
