3v0r: Difference between revisions
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==Crystal structure of Alternaria alternata allergen Alt a 1== | |||
=== | <StructureSection load='3v0r' size='340' side='right' caption='[[3v0r]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3v0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Alternaria_alternata Alternaria alternata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V0R FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FU:2,5,6-TRIAMINOPYRIMIDIN-4-OL'>0FU</scene>, <scene name='pdbligand=8AC:8-AMINOOCTANOIC+ACID'>8AC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALTA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5599 Alternaria alternata])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v0r RCSB], [http://www.ebi.ac.uk/pdbsum/3v0r PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
BACKGROUND: Alternaria species is one of the most common molds associated with allergic diseases, and 80% of Alternaria species-sensitive patients produce IgE antibodies to a major protein allergen, Alt a 1. The structure and function of Alt a 1 is unknown. OBJECTIVE: We sought to obtain a high-resolution structure of Alt a 1 using x-ray crystallography and to investigate structural relationships between Alt a 1 and other allergens and proteins reported in the Protein Data Bank. METHODS: X-ray crystallography was used to determine the structure of Alt a 1 by using a custom-designed set of crystallization conditions. An initial Alt a 1 model was determined by the application of a Ta(6)Br(12)(2+) cluster and single-wavelength anomalous diffraction. Bioinformatic analyses were used to compare the Alt a 1 sequence and structure with that of other proteins. RESULTS: Alt a 1 is a unique beta-barrel comprising 11 beta-strands and forms a "butterfly-like" dimer linked by a single disulfide bond with a large (1345 A(2)) dimer interface. Intramolecular disulfide bonds are conserved among Alt a 1 homologs. Currently, the Alt a 1 structure has no equivalent in the Protein Data Bank. Bioinformatics analyses suggest that the structure is found exclusively in fungi. Four previously reported putative IgE-binding peptides have been located on the Alt a 1 structure. CONCLUSIONS: Alt a 1 has a unique, dimeric beta-barrel structure that appears to define a new protein family with unknown function found exclusively in fungi. The location of IgE antibody-binding epitopes is in agreement with the structural analysis of Alt a 1. The Alt a 1 structure will allow mechanistic structure/function studies and immunologic studies directed toward new forms of immunotherapy for Alternaria species-sensitive allergic patients. | |||
Alternaria alternata allergen Alt a 1: A unique beta-barrel protein dimer found exclusively in fungi.,Chruszcz M, Chapman MD, Osinski T, Solberg R, Demas M, Porebski PJ, Majorek KA, Pomes A, Minor W J Allergy Clin Immunol. 2012 Jun 2. PMID:22664167<ref>PMID:22664167</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Alternaria alternata]] | [[Category: Alternaria alternata]] | ||
[[Category: Chapman, M D | [[Category: Chapman, M D]] | ||
[[Category: Chruszcz, M | [[Category: Chruszcz, M]] | ||
[[Category: Minor, W | [[Category: Minor, W]] | ||
[[Category: Osinski, T | [[Category: Osinski, T]] | ||
[[Category: Solberg, R | [[Category: Solberg, R]] | ||
[[Category: Beta-barrel]] | [[Category: Beta-barrel]] | ||
[[Category: Cell wall]] | [[Category: Cell wall]] | ||
[[Category: Unknown function]] | [[Category: Unknown function]] | ||
