3rip: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)== | |||
<StructureSection load='3rip' size='340' side='right' caption='[[3rip]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3rip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TUBGCP4, 76P, GCP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rip RCSB], [http://www.ebi.ac.uk/pdbsum/3rip PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Microtubule nucleation in all eukaryotes involves gamma-tubulin small complexes (gammaTuSCs) that comprise two molecules of gamma-tubulin bound to gamma-tubulin complex proteins (GCPs) GCP2 and GCP3. In many eukaryotes, multiple gammaTuSCs associate with GCP4, GCP5 and GCP6 into large gamma-tubulin ring complexes (gammaTuRCs). Recent cryo-EM studies indicate that a scaffold similar to gammaTuRCs is formed by lateral association of gammaTuSCs, with the C-terminal regions of GCP2 and GCP3 binding gamma-tubulin molecules. However, the exact role of GCPs in microtubule nucleation remains unknown. Here we report the crystal structure of human GCP4 and show that its C-terminal domain binds directly to gamma-tubulin. The human GCP4 structure is the prototype for all GCPs, as it can be precisely positioned within the gammaTuSC envelope, revealing the nature of protein-protein interactions and conformational changes regulating nucleation activity. | |||
Crystal structure of gamma-tubulin complex protein GCP4 provides insight into microtubule nucleation.,Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L Nat Struct Mol Biol. 2011 Jul 3;18(8):915-9. doi: 10.1038/nsmb.2083. PMID:21725292<ref>PMID:21725292</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | ==See Also== | ||
*[[Tubulin|Tubulin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Gregory-Pauron, L | [[Category: Gregory-Pauron, L]] | ||
[[Category: Guillet, V | [[Category: Guillet, V]] | ||
[[Category: Mourey, L | [[Category: Mourey, L]] | ||
[[Category: Gamma-tubulin ring complex]] | [[Category: Gamma-tubulin ring complex]] | ||
[[Category: Gamma-turc]] | [[Category: Gamma-turc]] | ||
[[Category: Helix bundle]] | [[Category: Helix bundle]] | ||
[[Category: Structural protein]] | [[Category: Structural protein]] | ||
