4g2t: Difference between revisions
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==Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate== | |||
<StructureSection load='4g2t' size='340' side='right' caption='[[4g2t]], [[Resolution|resolution]] 2.41Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4g2t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._sf2575 Streptomyces sp. sf2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G2T FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fzr|4fzr]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssfS6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746675 Streptomyces sp. SF2575])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g2t RCSB], [http://www.ebi.ac.uk/pdbsum/4g2t PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 A resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.Proteins 2013. (c) 2013 Wiley Periodicals, Inc. | |||
Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.,Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584<ref>PMID:23526584</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Streptomyces sp. sf2575]] | [[Category: Streptomyces sp. sf2575]] | ||
[[Category: Bingman, C A | [[Category: Bingman, C A]] | ||
[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]] | ||
[[Category: Phillips, G N | [[Category: Phillips, G N]] | ||
[[Category: Singh, S | [[Category: Singh, S]] | ||
[[Category: Thorson, J S | [[Category: Thorson, J S]] | ||
[[Category: Wang, F | [[Category: Wang, F]] | ||
[[Category: Zhou, M | [[Category: Zhou, M]] | ||
[[Category: Enzyme discovery for natural product biosynthesis]] | [[Category: Enzyme discovery for natural product biosynthesis]] | ||
[[Category: Glycosyltransferase]] | [[Category: Glycosyltransferase]] | ||
[[Category: Natpro]] | [[Category: Natpro]] | ||
[[Category: Protein structure initiative]] | [[Category: PSI, Protein structure initiative]] | ||
[[Category: Psi-biology]] | [[Category: Psi-biology]] | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
