2i2r: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2i2r.jpg|left|200px]] | [[Image:2i2r.jpg|left|200px]] | ||
'''Crystal structure of the KChIP1/Kv4.3 T1 complex''' | {{Structure | ||
|PDB= 2i2r |SIZE=350|CAPTION= <scene name='initialview01'>2i2r</scene>, resolution 3.35Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | |||
|ACTIVITY= | |||
|GENE= Kcnd3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), KCNIP1, KCHIP1, VABP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Crystal structure of the KChIP1/Kv4.3 T1 complex''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2I2R is a [ | 2I2R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2R OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer., Pioletti M, Findeisen F, Hura GL, Minor DL Jr, Nat Struct Mol Biol. 2006 Nov;13(11):987-95. Epub 2006 Oct 22. PMID:[http:// | Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer., Pioletti M, Findeisen F, Hura GL, Minor DL Jr, Nat Struct Mol Biol. 2006 Nov;13(11):987-95. Epub 2006 Oct 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057713 17057713] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 26: | Line 35: | ||
[[Category: potassium channel]] | [[Category: potassium channel]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:24:46 2008'' | ||
Revision as of 15:24, 20 March 2008
| |||||||||||||
| 2i2r, resolution 3.35Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | CA, ZN and NA | ||||||||||||
| Gene: | Kcnd3 (Rattus norvegicus), KCNIP1, KCHIP1, VABP (Homo sapiens) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the KChIP1/Kv4.3 T1 complex
Overview
Brain I(A) and cardiac I(to) currents arise from complexes containing Kv4 voltage-gated potassium channels and cytoplasmic calcium-sensor proteins (KChIPs). Here, we present X-ray crystallographic and small-angle X-ray scattering data that show that the KChIP1-Kv4.3 N-terminal cytoplasmic domain complex is a cross-shaped octamer bearing two principal interaction sites. Site 1 comprises interactions between a unique Kv4 channel N-terminal hydrophobic segment and a hydrophobic pocket formed by displacement of the KChIP H10 helix. Site 2 comprises interactions between a T1 assembly domain loop and the KChIP H2 helix. Functional and biochemical studies indicate that site 1 influences channel trafficking, whereas site 2 affects channel gating, and that calcium binding is intimately linked to KChIP folding and complex formation. Together, the data resolve how Kv4 channels and KChIPs interact and provide a framework for understanding how KChIPs modulate Kv4 function.
About this Structure
2I2R is a Protein complex structure of sequences from Homo sapiens and Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer., Pioletti M, Findeisen F, Hura GL, Minor DL Jr, Nat Struct Mol Biol. 2006 Nov;13(11):987-95. Epub 2006 Oct 22. PMID:17057713
Page seeded by OCA on Thu Mar 20 17:24:46 2008
